Anticorps Polyclonal de lapin anti-iPLA2

iPLA2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 22030-1-AP

Synonymes

CaI PLA2, Group VI phospholipase A2, GVI, GVI PLA2, INAD1, iPLA2, iPLA2 beta, IPLA2 VIA, PARK14, PLA2, PLA2G6, PNPLA9

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Galerie de données de validation

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  WB analysis of mouse testis using 22030-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22030-1-AP (iPLA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 22030-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22030-1-AP (iPLA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 22030-1-AP

  IP Result of anti-iPLA2 (IP:22030-1-AP, 4ug; Detection:22030-1-AP 1:800) with mouse testis tissue lysate 4000ug.

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  IHC staining of human small intestine using 22030-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22030-1-AP (iPLA2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 22030-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22030-1-AP (iPLA2 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 22030-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22030-1-AP (iPLA2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 22030-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22030-1-AP (iPLA2 antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

22030-1-AP cible iPLA2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: iPLA2 Protéine recombinante Ag16896
• Nom complet: phospholipase A2, group VI (cytosolic, calcium-independent)
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 80 kDa, 88 kDa
• Numéro d’acquisition GenBank: BC036742
• Symbole du gène: PLA2G6
• Identification du gène (NCBI): 8398
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLA2G6, also named as PLPLA9, is involved in the remodeling of membranes allowing arachidonic acid to be placed in the proper position in phospholipids for stimulated release. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. This protein has 4 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Cell Rep; Alpha synuclein determines ferroptosis sensitivity in dopaminergic neurons via modulation of ether-phospholipid membrane composition; Authors - Laura Mahoney-Sanchez
human,mouse	WB,IHC	J Agric Food Chem; Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism.; Authors - Shuhua Shan
rat	IF	Neurol Res; Calcium-independent phospholipase A2 inhibitor produces an analgesic effect in a rat model of neuropathic pain by reducing central sensitization in the dorsal horn.; Authors - Young Seob Gwak
human	IHC	Zhongguo Dang Dai Er Ke Za Zhi; [Clinical features of infantile neuroaxonal dystrophy and PLA2G6 gene testing].; Authors - Yao Lu
human	WB,IHC	Theranostics; Ferritin light chain promotes the reprogramming of glioma immune microenvironment and facilitates glioma progression; Authors - Hongjiang Li
human	IHC	Molecules; Identification of Diagnostic Metabolic Signatures in Thyroid Tumors Using Mass Spectrometry Imaging; Authors - Xinxin Mao