Anticorps Polyclonal de lapin anti-PAFAH

• Phare
• Validé par KD/KO

PAFAH Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 15526-1-AP

Synonymes

Group VIIA phospholipase A2, gVIIA PLA2, LDL PLA(2), LDL PLA2, Lp-PLA2, PAF 2 acylhydrolase, PAF acetylhydrolase, PAFAH, PAF-AH, PLA2G7

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Galerie de données de validation

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  WB analysis of RAW264.7 using 15526-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 15526-1-AP (PAFAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 15526-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15526-1-AP (PAFAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15526-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15526-1-AP (PAFAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 15526-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 15526-1-AP (PAFAH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 15526-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15526-1-AP (PAFAH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15526-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15526-1-AP (PAFAH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15526-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15526-1-AP (PAFAH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15526-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15526-1-AP (PAFAH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 15526-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed RAW 264.7 cells using 15526-1-AP (PAFAH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, RAW264.7, tissu hépatique de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 2 publications below

Informations sur le produit

15526-1-AP cible PAFAH dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAFAH Protéine recombinante Ag7227
• Nom complet: phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 43-67 kDa
• Numéro d’acquisition GenBank: BC038452
• Symbole du gène: PLA2G7
• Identification du gène (NCBI): 7941
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAFAH(platelet-activating factor acetylhydrolase, plasma) is also named as PLA2G7(phospholipase A2, group VII), Lp-PLA2 (Lipoprotein-associated phospholipase A2), LDL-PLA2, LDL-PLA and belongs to the AB hydrolase superfamily and lipase family. It is an enzyme which catalyzes the hydrolysis of acetyl ester at the sn-2 position of PAF. The family of PAF-Ahs consists of two intracellular isoforms (Ib and II), and one secreted isoform (plasma). Plasma PAF-AH is a monomeric polypeptide with a molecular weight of 45 kDa and it can show broad bands with a molecular weight of 43-67 kDa that it can be extensively N-glycosylated(PMID:12547653). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PAFAD), susceptibility to asthma (ASTHMA), susceptibility to atopic hypersensitivity (ATOPY).

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation.; Authors - Das Amrita A
mouse	WB	Nat Commun; VDAC2 enables BAX to mediate apoptosis and limit tumor development.; Authors - Hui San Chin
mouse	WB	J Extracell Vesicles; Characterization of protein, long noncoding RNA and microRNA signatures in extracellular vesicles derived from resting and degranulated mast cells.; Authors - Yuting Liang
mouse	IF,WB	Nat Metab; Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions.; Authors - Jacob T Boyd ; KD Validated
human	IF	Cell Death Discov; Characterizing cellular heterogeneity in fibrotic hypersensitivity pneumonitis by single-cell transcriptional analysis.; Authors - Junyi Wang
human	WB	Int J Mol Sci; PLA2G7/PAF-AH as Potential Negative Regulator of the Wnt Signaling Pathway Mediates Protective Effects in BRCA1 Mutant Breast Cancer; Authors - Yue Liao ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sathyanarayani (Verified Customer) (05-12-2023)

I used this PAFAH antibody at a range of different dilutions from 1:300 to 1:1000 but unfortunately I could not detect any bands from this antibody when I used lysed mouse blood. The loading controls which were also from Proteintech produced strong clear bands on the western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Lysed mouse blood