Anticorps Polyclonal de lapin anti-PLAC8

• Phare
• Validé par KD/KO

PLAC8 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 12284-1-AP

Synonymes

BM-004, C15, onzin, placenta 8, Placenta-specific gene 8 protein

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Galerie de données de validation

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  WB analysis of human peripheral blood leukocyte using 12284-1-AP

  human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 12284-1-AP (PLAC8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 12284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12284-1-AP (PLAC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12284-1-AP (PLAC8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12284-1-AP (PLAC8 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: leucocyte de sang périphérique humain, PANC-1 cells
• Résultats positifs en IHC: tissu placentaire humain, tissu cérébral humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• CoIP: See 2 publications below

Informations sur le produit

12284-1-AP cible PLAC8 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLAC8 Protéine recombinante Ag2939
• Nom complet: placenta-specific 8
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC012205
• Symbole du gène: PLAC8
• Identification du gène (NCBI): 51316
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLAC8, also named as Protein C15, is a placenta-specific protein. The expression was downregulated upon activation, particularly in dendritic cells generated in vitro from CD34 (142230) progenitor cells. The MW of PLAC8 is about 12-20kd.

Publications

Species	Application	Title
human	IHC	FASEB J; PLAC8 promotes the autophagic activity and improves the growth priority of human trophoblast cells.; Authors - Xuan Feng
human	IHC	Int J Med Sci; Expression profiling of lncRNAs and mRNAs in placental site trophoblastic tumor (PSTT) by microarray.; Authors - Jianfeng Gan
human	WB,IHC	Histochem Cell Biol; PLAC8 contributes to the malignant behaviors of cervical cancer cells by activating the SOX4-mediated AKT pathway; Authors - Boya Deng ; KD Validated
human	IF	PLoS Genet; Single-cell RNA profiling reveals classification and characteristics of mononuclear phagocytes in colorectal cancer; Authors - Tiantian Ji
human	WB,IHC	Front Immunol; Identification and validation of immune and diagnostic biomarkers for interstitial cystitis/painful bladder syndrome by integrating bioinformatics and machine-learning; Authors - Tao Zhou
human,mouse	WB,CoIP	Commun Biol; PLAC8 attenuates pulmonary fibrosis and inhibits apoptosis of alveolar epithelial cells via facilitating autophagy; Authors - Wei Sun

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