Anticorps Monoclonal anti-PLAP

• Phare
• Validé par KD/KO

PLAP Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(9)

CloneNo.

2B7C9

N° de cat : 60294-1-Ig

Synonymes

PLAP-1, PALP, EC:3.1.3.1, ALPP, ALP

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Galerie de données de validation

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  WB analysis of HepG2 using 60294-1-Ig

  WB result of PLAP antibody (60294-1-Ig, 1:1000) with si-control and si-PLAP transfected HepG2 cells.

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  WB analysis of HepG2 using 60294-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60294-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 60294-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 60294-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 60294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 60294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 60294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 10x lens).

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  IHC staining of human malignant melanoma using 60294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 40x lens).

• 

  IHC staining of human placenta using 60294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 60294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 60294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: SCaBER cells, cellules HepG2, tissu placentaire humain
• Résultats positifs en IHC: tissu placentaire humain, tissu d'appendicite humain, tissu de mélanome malin humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IF: See 2 publications below

Informations sur le produit

60294-1-Ig cible PLAP dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PLAP Protéine recombinante Ag13361
• Nom complet: alkaline phosphatase, placental (Regan isozyme)
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC009647
• Symbole du gène: PLAP
• Identification du gène (NCBI): 250
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.

Publications

Species	Application	Title
mouse	WB,IF	J Agric Food Chem; Sodium Benzoate Inhibits Osteoblast Differentiation and Accelerates Bone Loss by Regulating the FGF2/p38/RUNX2 Pathway; Authors - Zhonghao Wang
rat	IF	J Cell Mol Med; Glucocorticoids decreased Cx43 expression in osteonecrosis of femoral head: The effect on proliferation and osteogenic differentiation of rat BMSCs.; Authors - Xin Zhao
rat	WB	Front Bioeng Biotechnol; Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects; Authors - Situo Wang
rat	WB	Biomed Pharmacother; The multi-protective effect of IL-37-Smad3 against ox-LDL induced dysfunction of endothelial cells; Authors - Changyi Zhang
rat	WB	J Biomed Mater Res A; Copper ions-photo dual-crosslinked alginate hydrogel for angiogenesis and osteogenesis; Authors - Guochen Liu
mouse	WB	Cell Death Differ; Foxk1 promotes bone formation through inducing aerobic glycolysis; Authors - Chungeng Liu

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