Anticorps Monoclonal anti-PLAP

• Phare
• Validé par KD/KO

PLAP Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

2B7C9

N° de cat : 60294-1-Ig

Synonymes

ALP, ALPP, PALP, Placental alkaline phosphatase, PLAP, PLAP 1

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Galerie de données de validation

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  WB analysis of HepG2 using 60294-1-Ig

  WB result of PLAP antibody (60294-1-Ig, 1:1000) with si-control and si-PLAP transfected HepG2 cells.

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  WB analysis of HepG2 using 60294-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60294-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 60294-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 60294-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 60294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 60294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 60294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 60294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 40x lens).

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  FC experiment of HepG2 using 60294-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human PLAP (60294-1-Ig, Clone:2B7C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: SCaBER cells, cellules HepG2, tissu placentaire humain
• Résultats positifs en IHC: tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

60294-1-Ig cible PLAP dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PLAP Protéine recombinante Ag13361
• Nom complet: alkaline phosphatase, placental (Regan isozyme)
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC009647
• Symbole du gène: ALPP
• Identification du gène (NCBI): 250
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.

Publications

Species	Application	Title
rat	IF	J Cell Mol Med; Glucocorticoids decreased Cx43 expression in osteonecrosis of femoral head: The effect on proliferation and osteogenic differentiation of rat BMSCs.; Authors - Xin Zhao
rat	WB	Front Bioeng Biotechnol; Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects; Authors - Situo Wang