Anticorps Polyclonal de lapin anti-Phospholipase C Beta 1

• Phare
• Validé par KD/KO

Phospholipase C Beta 1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, RIP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 26551-1-AP

Synonymes

KIAA0581, Phospholipase C beta 1, Phospholipase C I, PI PLC, PLC 154, PLC beta 1, PLC I, PLC154, PLCB1

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Galerie de données de validation

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  WB analysis of mouse brain using 26551-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26551-1-AP (Phospholipase C beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 26551-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26551-1-AP (Phospholipase C beta 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 26551-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26551-1-AP (Phospholipase C beta 1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 26551-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26551-1-AP (Phospholipase C beta 1 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 26551-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26551-1-AP (Phospholipase C beta 1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 26551-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26551-1-AP (Phospholipase C beta 1 antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu de gliome humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

26551-1-AP cible Phospholipase C Beta 1 dans les applications de WB, IHC, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Phospholipase C Beta 1 Protéine recombinante Ag24750
• Nom complet: phospholipase C, beta 1 (phosphoinositide-specific)
• Masse moléculaire calculée: 1216 aa, 139 kDa
• Poids moléculaire observé: 139 kDa
• Numéro d’acquisition GenBank: BC069420
• Symbole du gène: PLCB1
• Identification du gène (NCBI): 23236
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLCB1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. PLCB1 is activated by two G-protein alpha subunits, alpha-q and alpha-11.

Publications

Species	Application	Title
human	WB	Biochem Genet; PLCB1 Enhances Cell Migration and Invasion in Gastric Cancer Via Regulating Actin Cytoskeletal Remodeling and Epithelial-Mesenchymal Transition; Authors - Yulin Wang ; KD Validated
human	WB	Front Endocrinol (Lausanne); Estrogen receptor subtype mediated anti-inflammation and vasorelaxation via genomic and nongenomic actions in septic mice; Authors - Luyun Zhang
human	WB,IHC	Nat Chem Biol; ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism; Authors - Linchong Sun ; KD Validated
sheep	WB	Int J Mol Sci; Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway; Authors - Yu Liang
human	WB,RIP	Thorac Cancer; LncRNA AC100826.1 regulated PLCB1 to promote progression in non-small cell lung cancer; Authors - Shenhui Dai
rat,human	WB,IHC	J Ethnopharmacol; Dan-Shen-Yin against ethanol-induced chronic gastric mucosal injury in rats by inhibiting apoptosis via IP3R-controlled calcium release; Authors - Zhirong Zhou

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