Anticorps Polyclonal de lapin anti-PLCB3

PLCB3 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 51139-1-AP

Synonymes

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3, EC:3.1.4.11, Phosphoinositide phospholipase C-beta-3, Phospholipase C beta 3, Phospholipase C-beta-3

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Galerie de données de validation

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  WB analysis of HeLa using 51139-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51139-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 51139-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 51139-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 51139-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 51139-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of MDA-MB-231 using 51139-1-AP

  IP result of anti-PLCB3 (IP:51139-1-AP, 4ug; Detection:51139-1-AP 1:1000) with MDA-MB-231 cells lysate 1280 ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293T, cellules MCF-7
• Résultats positifs en IP: cellules MDA-MB-231,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

51139-1-AP cible PLCB3 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: phospholipase C, beta 3 (phosphatidylinositol-specific)
• Masse moléculaire calculée: 1234 aa, 139 kDa
• Poids moléculaire observé: 139 kDa
• Numéro d’acquisition GenBank: BC142681
• Symbole du gène: PLCB3
• Identification du gène (NCBI): 5331
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.

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