Anticorps Polyclonal de lapin anti-PLEK2

• Phare
• Validé par KD/KO

PLEK2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 11685-1-AP

Synonymes

pleckstrin 2, PLEK2

---

Galerie de données de validation

• 

  WB analysis using 11685-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11685-1-AP

  U2OS (positive), Jurkat, Ramos, and U-937 (negative) cells were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 11685-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11685-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11685-1-AP (PLEK2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse spleen using 11685-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11685-1-AP (PLEK2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse spleen using 11685-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11685-1-AP (PLEK2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HT-29 using 11685-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using PLEK2 antibody (11685-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HT-29 using 11685-1-AP

  1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human PLEK2 (11685-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules HT-29, cellules Caco-2, cellules PC-3, cellules U2OS
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HT-29,
• Résultats positifs en cytométrie: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 12 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11685-1-AP cible PLEK2 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLEK2 Protéine recombinante Ag2297
• Nom complet: pleckstrin 2
• Masse moléculaire calculée: 353 aa, 40 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC001226
• Symbole du gène: PLEK2
• Identification du gène (NCBI): 26499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IHC	J Clin Invest; Loss of pleckstrin-2 reverts lethality and vascular occlusions in JAK2V617F-positive myeloproliferative neoplasms.; Authors - Baobing Zhao ; KO Validated
human	WB,IHC	Cancer Lett; Pleckstrin-2 promotes tumour immune escape from NK cells by activating the MT1-MMP-MICA signalling axis in gastric cancer; Authors - Deli Mao ; KD Validated
mouse	WB,IF	Haematologica; Targeted shRNA screening identified critical roles of pleckstrin-2 in erythropoiesis.; Authors - Baobing Zhao ; KD Validated
human	WB,IHC,IF	Mol Carcinog; PLEK2 mediates metastasis and invasion via α5-nAChR activation in nicotine-induced lung adenocarcinoma; Authors - Qiang Li ; KD Validated
mouse	WB, IF	Commun Biol; Pleckstrin-2 is essential for erythropoiesis in β-thalassemic mice, reducing apoptosis and enhancing enucleation.; Authors - Maria Feola
human	WB,IHC	Cell Signal; microRNA-873 inhibits self-renewal and proliferation of pancreatic cancer stem cells through pleckstrin-2-dependent PI3K/AKT pathway.; Authors - Xiao-Li Yang ; KD Validated