Anticorps Polyclonal de lapin anti-PLEKHF1

• Phare
• Validé par KD/KO

PLEKHF1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 20389-1-AP

Synonymes

Apoptosis inducing protein, APPD, LAPF, Phafin 1, PHAFIN1, PLEKHF1, ZFYVE15

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 20389-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 20389-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 20389-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 20389-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 20389-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 20389-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 20389-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 20389-1-AP (PLEKHF1 Antibody) at dilution of 1:50.

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  IHC staining of human brain using 20389-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 20389-1-AP (PLEKHF1 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 20389-1-AP

  Immunofluorescent analysis of HepG2 cells, using PLEKHF1 antibody 20389-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique humain, tissu de muscle squelettique de souris, tissu de thymus de souris, tissu pulmonaire de souris, tissu rénal humain, tissu splénique de souris
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

20389-1-AP cible PLEKHF1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLEKHF1 Protéine recombinante Ag14228
• Nom complet: pleckstrin homology domain containing, family F (with FYVE domain) member 1
• Masse moléculaire calculée: 279 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC002744
• Symbole du gène: PLEKHF1
• Identification du gène (NCBI): 79156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLEKHF1, also named as APPD, LAPF, and ZFYVE15, is a 279 amino acid protein, which contains one PH domain and one FYVE-type zinc finger. PLEKHF1 translocates to lysosome during apoptosis, localizing in the nucleus and cytoplsam. PLEKHF1 is highly expressed in heart and skeletal muscle and weakly expressed in brain, thymus, spleen, kidney, liver, small intestine, placenta and lung. PLEKHF1 may induce apoptosis through the lysosomal-mitochondrial pathway. PLEKHF1 modulates the protein content of the plasma membrane and was involved in different endocytosis events.

Publications

Species	Application	Title
human	WB	Breast Cancer Res; Functional characterization of the 19q12 amplicon in grade III breast cancers.; Authors - Natrajan Rachael R ; KD Validated
mouse	WB,IF	Sci China Life Sci; Local administration of liposomal-based Plekhf1 gene therapy attenuates pulmonary fibrosis by modulating macrophage polarization; Authors - Lifeng Yan ; KD Validated