Anticorps Polyclonal de lapin anti-PLK2

• Phare
• Validé par KD/KO

PLK2 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP,ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15956-1-AP

Synonymes

PLK 2, PLK2, Polo like kinase 2, Serum inducible kinase, SNK

---

Galerie de données de validation

• 

  WB analysis using 15956-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15956-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15956-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15956-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15956-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15956-1-AP

  IP Result of anti-PLK2 (IP:15956-1-AP, 4ug; Detection:15956-1-AP 1:300) with HeLa cells lysate 2480ug.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules MCF-7
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

15956-1-AP cible PLK2 dans les applications de WB, IP,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLK2 Protéine recombinante Ag8710
• Nom complet: polo-like kinase 2 (Drosophila)
• Masse moléculaire calculée: 685 aa, 78 kDa
• Poids moléculaire observé: 75-80 kDa
• Numéro d’acquisition GenBank: BC013879
• Symbole du gène: PLK2
• Identification du gène (NCBI): 10769
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLK2(Polo-like kinase 2) is also named as SNK and belongs to the Ser/Thr protein kinase family. It plays a role in normal cell division. Plk2 expression is detected only in G1, and endogenous Plk2 is rapidly turned over. Therefore, the expression of the three mammalian Plks during the cell cycle resembles the successive transition of the cyclins, prompting speculation that Plks may reg-ulate the cell cycle in a manner analogous to that of Cdks(PMID:12972611).

Publications

Species	Application	Title
human	WB	Oncotarget; MicroRNA-27b up-regulated by human papillomavirus 16 E7 promotes proliferation and suppresses apoptosis by targeting polo-like kinase2 in cervical cancer.; Authors - Fei Liu
rat	WB	J Biochem Mol Toxicol; PLK2 protects retinal ganglion cells from oxidative stress by potentiating Nrf2 signaling via GSK-3β.; Authors - Yazhi Fan ; KD Validated
	IP	Cell Biol Int; Tazarotene-induced gene 1 interacts with Polo-like kinase 2 and inhibits cell proliferation in HCT116 colorectal cancer cells.; Authors - Chun-Hua Wang