Anticorps Polyclonal de lapin anti-PLK4

PLK4 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 28750-1-AP

Synonymes

PLK 4, PLK4, Polo like kinase 4, SAK, STK18

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Galerie de données de validation

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  IHC staining of human colon cancer using 28750-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 28750-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 28750-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28750-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28750-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 28750-1-AP (PLK4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

28750-1-AP cible PLK4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLK4 Protéine recombinante Ag30482
• Nom complet: polo-like kinase 4 (Drosophila)
• Masse moléculaire calculée: 970 aa, 109 kDa
• Numéro d’acquisition GenBank: BC036023
• Symbole du gène: PLK4
• Identification du gène (NCBI): 10733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLK4 (Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Biochem Biophys Res Commun; Advanced glycation end products initiate the mutual promoting cycle between centrosome amplification and the release of inflammatory cytokines in human vascular endothelial cells; Authors - Jun Zhang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Franziska (Verified Customer) (02-15-2023)

RPE1 WT cells were starved for 2 days. Fixed with -20°C Ethanol for 10 min at room temperature (RT), permeablized with 0.3% PBST(triton), blocked with 1%BSA in PBS for 1h at RT, PLK4 (rb, 1:50) and ARL13B (ms, 1:200) in 1% BSA in PBS diluted, prim AK Incubation for 1h at RT. Sek Ak goat anti rb (green) and goat anti mouse (pink). PLK4 can be found at the Basal Body of cilia and also stains cytoskeleton. PLK4 antibody could be tried with 1:200 dilution, to reduce background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50, maybe 1:200 better
• Cell Tissue Type: hTERT-RPE1

PLK4 Antibody Immunofluorescence validation (1:50, maybe 1:200 better dilution) in hTERT-RPE1 (Cat no:28750-1-AP)