Anticorps Polyclonal de lapin anti-PLS3

• Phare
• Validé par KD/KO

PLS3 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 55216-1-AP

Synonymes

Plastin 3, plastin 3 (T isoform), PLS3, T plastin

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Galerie de données de validation

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  WB analysis of HepG2 using 55216-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 55216-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 55216-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55216-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55216-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55216-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus using 55216-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus using 55216-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 55216-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55216-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules COLO 320, cellules HeLa
• Résultats positifs en IHC: tissu œsophagien humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

55216-1-AP cible PLS3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: plastin 3 (T isoform)
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 63 kDa, 38 kDa
• Numéro d’acquisition GenBank: NM_005032
• Symbole du gène: PLS3
• Identification du gène (NCBI): 5358
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLS3, also named as T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. Both T- and L-plastin are implicated in several diseases, L-plastin is considered to be a valuable marker for cancer. (PMID: 15960882) This antibody is specific to PLS3

Publications

Species	Application	Title
human	WB,IHC	J Cell Physiol; PLS3 predicts poor prognosis in pancreatic cancer and promotes cancer cell proliferation via PI3K/AKT signaling.; Authors - Zechang Xin ; KD Validated
human	WB,IHC	Environ Toxicol; PLS3 promotes papillary thyroid carcinoma progression by activating the Notch signaling pathway; Authors - Dongtao Wang ; KD Validated
human	WB	J Cell Biochem; Phosphorylation of mitochondrial phospholipid scramblase 3 by protein kinase C-delta induces its activation and facilitates mitochondrial targeting of tBid.; Authors - Yongwen He ; KD Validated
mouse	WB	Biochim Biophys Acta; Comparative proteomic analysis of mouse models of pathological and physiological cardiac hypertrophy, with selection of biomarkers of pathological hypertrophy by integrative Proteogenomics.; Authors - Hye Kyeong Kwon