Anticorps Polyclonal de lapin anti-PLVAP

PLVAP Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 14452-1-AP

Synonymes

FELS, Fenestrated endothelial-linked structure protein, gp68, Plasmalemma vesicle-associated protein, PV 1

---

Galerie de données de validation

• 

  WB analysis using 14452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14452-1-AP (PLVAP antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules L02, tissu hépatique de rat, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:9000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

14452-1-AP cible PLVAP dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLVAP Protéine recombinante Ag5963
• Nom complet: plasmalemma vesicle associated protein
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC056414
• Symbole du gène: PLVAP
• Identification du gène (NCBI): 83483
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Plasmalemma vesicle-associated protein (PLVAP) is an endothelial-associated protein that is involved in endothelial diaphragm formation and maintenance of basal vascular permeability. At the same time, PLVAP has roles in immunity by facilitating leukocyte diapedesis at inflammatory sites and controlling peripheral lymph node morphogenesis and the entry into soluble ags into lymph node conduits .(PMID: 36884119, PMID: 37810232)

Publications

Species	Application	Title
mouse	WB	Neuron; The Wnt Inhibitor Apcdd1 Coordinates Vascular Remodeling and Barrier Maturation of Retinal Blood Vessels.; Authors - Jenna Mazzoni
pig	WB	J Virol; Porcine Epidemic Diarrhea Virus Infection Disrupts the Nasal Endothelial Barrier To Favor Viral Dissemination.; Authors - Jianda Li
human	WB	Front Pharmacol; Stimulatory G-Protein α Subunit Modulates Endothelial Cell Permeability Through Regulation of Plasmalemma Vesicle-Associated Protein.; Authors - Lifan He
mouse	WB	Biochem Biophys Res Commun; Retinal VEGFA maintains the ultrastructure and function of choriocapillaris by preserving the endothelial PLVAP.; Authors - Sang A Kim
		bioRxiv; Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signaling; Authors - Saptarshi Biswas
mouse	WB	Neuron; Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signaling; Authors - Saptarshi Biswas

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Michelle (Verified Customer) (12-01-2023)

This antibody works very well for PLVAP westernblot analysis. We detected strong und specific PLVAP bands, even with this relatively high dilution of 1:4500.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4500
• Cell Tissue Type: cultured bEnd5 cells

{{ptg:RelatedPrimaryAntibodies}}