Anticorps Polyclonal de lapin anti-PLXNB1

PLXNB1 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 23795-1-AP

Synonymes

KIAA0407, plexin B1, PLXN5, PLXNB1, Semaphorin receptor SEP, SEP

---

Galerie de données de validation

• 

  WB analysis of mouse brain using 23795-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23795-1-AP (PLXNB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 23795-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23795-1-AP (PLXNB1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 23795-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23795-1-AP (PLXNB1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 23795-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23795-1-AP (PLXNB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 23795-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23795-1-AP (PLXNB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 23795-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug PLXNB1 antibody (23795-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

23795-1-AP cible PLXNB1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLXNB1 Protéine recombinante Ag20719
• Nom complet: plexin B1
• Masse moléculaire calculée: 2135 aa, 232 kDa
• Poids moléculaire observé: 210 kDa
• Numéro d’acquisition GenBank: BC146793
• Symbole du gène: PLXNB1
• Identification du gène (NCBI): 5364
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB1 is one of the three plexin-B family members. It is a high-affinity receptor for semaphorin 4D (SEMA4D). Interaction of SEMA4D and PLXNB1 has important physiologic effects in the immune and nervous systems, and is also involved in tumor progression, especially in tumor angiogenesis (PMID: 20858260).

Publications

Species	Application	Title
mouse	FC	Cancer Immunol Res; Semaphorin4D Inhibition Improves Response to Immune-Checkpoint Blockade via Attenuation of MDSC Recruitment and Function.; Authors - Paul E Clavijo
human	WB	J Transl Med; SEMA4D/PlexinB1 promotes AML progression via activation of PI3K/Akt signaling.; Authors - Lu Liu
rat	WB	Ann Transl Med; Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage.; Authors - Kun Wang
mouse	IF	J Neuroendocrinol; PLXNB1 mutations in the etiology of idiopathic hypogonadotropic hypogonadism.; Authors - Bradley A Welch
human	IHC	Ann Transl Med; Methylation-based epigenetic studies and gene integration analysis of preeclampsia; Authors - Lei Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Boyan (Verified Customer) (10-04-2021)

calculated size 230 kd. this antibody recognized major bands at 35, 30 kd.

• Applications: Western Blot