Anticorps Polyclonal de lapin anti-Plexin B2

• Phare
• Validé par KD/KO

Plexin B2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10602-1-AP

Synonymes

KIAA0315, MM1, Nbla00445, PLEXB2, Plexin B2, PLXNB2

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Galerie de données de validation

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  WB analysis of A549 using 10602-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10602-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 10602-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10602-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10602-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10602-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10602-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10602-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10602-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10602-1-AP

  IP Result of anti-Plexin B2 (IP:10602-1-AP, 4ug; Detection:10602-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human tonsillitis using 10602-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10602-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10602-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10602-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10602-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10602-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10602-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10602-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10602-1-AP

  Immunofluorescent analysis of HepG2 cells using 10602-1-AP (Plexin B2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, tissu cérébral humain, tissu hépatique de souris, tissu ovarien de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du foie humain, tissu de cancer du sein humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

10602-1-AP cible Plexin B2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Plexin B2 Protéine recombinante Ag0941
• Nom complet: plexin B2
• Masse moléculaire calculée: 205 kDa
• Poids moléculaire observé: 80 kDa, 240 kDa
• Numéro d’acquisition GenBank: BC004542
• Symbole du gène: PLXNB2
• Identification du gène (NCBI): 23654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 1542-1841aa of human plexin B2 can recognize precursor and beta subunit of plexin B2.

Publications

Species	Application	Title
human	WB	Nat Commun; Osteoclasts protect bone blood vessels against senescence through the angiogenin/plexin-B2 axis.; Authors - Xiaonan Liu ; KD Validated
mouse	WB,IF	EMBO J; Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.; Authors - Rongpan Bai ; KD Validated
human	WB,IHC,IP	bioRxiv; Computational ranking-assisted identification of Plexin-B2 in homotypic and heterotypic clustering of circulating tumor cells in breast cancer metastasis; Authors - Emma Schuster ; KD Validated
mouse	IF	Sci Adv; A balance of noncanonical Semaphorin signaling from the cerebrospinal fluid regulates apical cell dynamics during corticogenesis; Authors - Katrin Gerstmann
mouse	IF	Hum Reprod; Whole exome sequencing in unexplained recurrent miscarriage families identified novel pathogenic genetic causes of euploid miscarriage; Authors - Xiyao Wang
mouse	WB	Mol Hum Reprod; Semaphorin 4C regulates ovarian steroidogenesis through RHOA/ROCK1-mediated actin cytoskeleton rearrangement; Authors - Dan Chen