Anticorps Monoclonal anti-PLZF

PLZF Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

2E6F12

N° de cat : 66672-1-Ig

Synonymes

ZBTB16, 2E6F12, Zinc finger and BTB domain-containing protein 16, Zinc finger protein 145, Zinc finger protein PLZF

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Galerie de données de validation

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  WB analysis of Ramos using 66672-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 66672-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66672-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66672-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66672-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66672-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66672-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Ramos, cellules Daudi, cellules HeLa, cellules Raji, cellules THP-1, tissu testiculaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

66672-1-Ig cible PLZF dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PLZF Protéine recombinante Ag25180
• Nom complet: zinc finger and BTB domain containing 16
• Masse moléculaire calculée: 673 aa, 74 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC026902
• Symbole du gène: PLZF
• Identification du gène (NCBI): 7704
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLZF, also named as Zinc finger protein 145, is a 673 amino acid protein, which belongs to the krueppel C2H2-type zinc-finger protein family. PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. PLZF may play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues.

Publications

Species	Application	Title
mouse	WB	Front Physiol; Abscopal effects of thoracic X-ray radiation on spermatogenesis in mice; Authors - Pan-Pan Lai
mouse	WB	iScience; Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability; Authors - Ziqian Min
mouse	WB	J Hazard Mater; Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice; Authors - Tianxin Zhao
mouse	IF	Med Microbiol Immunol; Effects of acetate-producing Blautia wexlerae on oxidative stress and NLRP3 inflammasome in obesity-associated male infertility; Authors - Yucheng Zhong
mouse	WB	Biomed J; Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism; Authors - Lin Chen

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