Anticorps Polyclonal de lapin anti-PMP2

PMP2 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 12717-1-AP

Synonymes

FABP8, M FABP, MP2, Myelin P2 protein, P2, peripheral myelin protein 2, PMP2

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Galerie de données de validation

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  WB analysis of pig spinal cord using 12717-1-AP

  pig spinal cord tissue were subjected to SDS PAGE followed by western blot with 12717-1-AP (PMP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12717-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12717-1-AP (PMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12717-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12717-1-AP (PMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12717-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12717-1-AP (PMP2 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu de moelle épinière de porc, tissu cérébral humain, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

12717-1-AP cible PMP2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PMP2 Protéine recombinante Ag3411
• Nom complet: peripheral myelin protein 2
• Masse moléculaire calculée: 132 aa, 15 kDa
• Poids moléculaire observé: 15-20 kDa
• Numéro d’acquisition GenBank: BC034997
• Symbole du gène: PMP2
• Identification du gène (NCBI): 5375
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PMP2 (peripheral myelin protein-2, also known as P2) is one of the major proteins of peripheral myelin and appears to be related to the transport of fatty acids or the metabolism of myelin lipids.P2 constitutes up to 15% of total protein of peripheral nervous system (PNS) myelin. It is also present in small amounts in central nervous system (CNS) myelin, being abundant in spinal cord and brain stem myelin. As a structural protein, P2 is thought to stabilize the myelin membranes.

Publications

Species	Application	Title
human	IHC	Nat Neurosci; Variation among intact tissue samples reveals the core transcriptional features of human CNS cell classes.; Authors - Kevin W Kelley
mouse	IHC	Nat Neurosci; Disentangling glial diversity in peripheral nerves at single-nuclei resolution.; Authors - Aldrin K Y Yim
pig	WB	Neuron; Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction.; Authors - Nadav I Weinstock
mouse	WB	Elife; Proteome profile of peripheral myelin in healthy mice and in a neuropathy model.; Authors - Sophie B Siems
human,mouse	WB,IHC	Elife; Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.; Authors - Vasiliki-Ilya Gargareta
mouse	IHC	Glia; A role of peripheral myelin protein 2 in lipid homeostasis of myelinating schwann cells.; Authors - Jennifer Zenker