Anticorps Polyclonal de lapin anti-Pinin

• Phare
• Validé par KD/KO

Pinin Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 18266-1-AP

Synonymes

Desmosome associated protein, Domain rich serine protein, DRS, DRS protein, DRSP, memA, Nuclear protein SDK3, Pinin, PNN, SDK3, SR like protein

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Galerie de données de validation

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  WB analysis using 18266-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18266-1-AP (Pinin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18266-1-AP

  IP Result of anti-Pinin (IP:18266-1-AP, 4ug; Detection:18266-1-AP 1:300) with HeLa cells lysate 520ug.

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  IF Staining of HeLa using 18266-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Pinin antibody (18266-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

18266-1-AP cible Pinin dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Pinin Protéine recombinante Ag6643
• Nom complet: pinin, desmosome associated protein
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 82 kDa, 140 kDa
• Numéro d’acquisition GenBank: BC062602
• Symbole du gène: PNN
• Identification du gène (NCBI): 5411
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PNN, also named as DRS or 140 kDa nuclear and cell adhesion-related phosphoprotein, is a 717 amino acid protein, which belongs to the pinin family. PNN localizes in the plasma membrane and is expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. PNN is expressed strongly in melanoma metastasis lesions and advanced primary tumors. PNN as a transcriptional activator binds to the E-box 1 core sequence of the E-cadherin promoter gene. PNN is involved in the establishment and maintenance of epithelia cell-cell adhesion. PNN is a potential tumor suppressor for renal cell carcinoma. The calculated molecular weight of PNN is 82 kDa (native form), but the post-modified protein is 140 kDa (phospho form of isoform 1).

Publications

Species	Application	Title
human	WB,RIP	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
human	WB,IP,IF	Mol Oncol; LncRNA AATBC regulates Pinin to promote metastasis in nasopharyngeal carcinoma.; Authors - Ting Tang ; KD Validated
human	WB	Oncotarget; Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.; Authors - Liyuan Zhang
human,mouse	WB,IHC	Exp Physiol; Methyltransferase like 3 enhances pinin mRNA stability through N6 -methyladenosine modification to augment tumourigenesis of colon adenocarcinoma; Authors - Min He
human	WB	Oncotarget; Pinin associates with prognosis of hepatocellular carcinoma through promoting cell proliferation and suppressing glucose deprivation-induced apoptosis.; Authors - Xuejun Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (05-14-2023)

ICC on HeLa cells; antibody diluted in 5% goat serum in PBST; 2 h at RT temperature incubation. Labels nuclear speckles. Very good signal

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela

Pinin Antibody Immunofluorescence validation (1:1000 dilution) in Hela (Cat no:18266-1-AP)