Anticorps Polyclonal de lapin anti-PNO1

• Phare
• Validé par KD/KO

PNO1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 21059-1-AP

Synonymes

KHRBP1, Partner of NOB1, RNA binding protein PNO1, RNA-binding protein PNO1

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Galerie de données de validation

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  WB analysis using 21059-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21059-1-AP (PNO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 21059-1-AP

  WB result of PNO1 antibody (21059-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-PNO1 transfected K-562 cells.

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  WB analysis of mouse spleen using 21059-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21059-1-AP (PNO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 21059-1-AP

  IP result of anti-PNO1 (IP:21059-1-AP, 3ug; Detection:21059-1-AP 1:300) with L02 cells lysate 1500ug.

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  IHC staining of human liver cancer using 21059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21059-1-AP (PNO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 21059-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21059-1-AP (PNO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 21059-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21059-1-AP (PNO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules A549, cellules K-562, tissu splénique de souris
• Résultats positifs en IP: cellules L02,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

21059-1-AP cible PNO1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PNO1 Protéine recombinante Ag14752
• Nom complet: partner of NOB1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 252 aa, 28 kDa
• Poids moléculaire observé: 28-35 kDa
• Numéro d’acquisition GenBank: BC008304
• Symbole du gène: PNO1
• Identification du gène (NCBI): 56902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human RNA binding gene partner of NOB1 (PNO1) was firstly isolated from human kidney and encodes 35-kDa protein, named PNO1. PNO1 as a ribosome biogenesis factor that contributes to 40S subunit assembly by binding with its partner NOB1. PNO1 is a highly conserved protein with a K homology (KH) domain at its C-terminal and two putative nuclear localization signals at its N-terminal . PNO1 also had great regulatory function in tumor metabolism. (PMID: 38071381, PMID: 33864661)

Publications

Species	Application	Title
human	WB	Sci Rep; PNO1 promotes the progression of osteosarcoma via TGF-β and YAP/TAZ pathway; Authors - Long Fang ; KD Validated
human	IHC	Heliyon; Pan-cancer exploration of PNO1: A prospective prognostic biomarker with ties to immune infiltration; Authors - Yinhui Qin
human	WB,IHC	Sci Rep; PNO1 enhances ovarian cancer cell growth, invasion, and stemness via activating the AKT/Wnt/β-catenin pathway; Authors - Lu Qin

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