Anticorps Polyclonal de lapin anti-Podocalyxin

• Phare
• Validé par KD/KO

Podocalyxin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 18150-1-AP

Synonymes

GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, Renal Cell Carcinoma marker, TRA 1 81,TRA 1 60, TRA-1-60

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Galerie de données de validation

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  WB analysis of HEK-293 using 18150-1-AP

  WB result of Podocalyxin antibody (18150-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Podocalyxin transfected HEK-293 cells.

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  WB analysis of human kidney using 18150-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 18150-1-AP (PODXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18150-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18150-1-AP (PODXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18150-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18150-1-AP (PODXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18150-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18150-1-AP (Podocalyxin antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18150-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18150-1-AP (Podocalyxin antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human kidney using 18150-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Podocalyxin antibody (18150-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Calbindin-D28k antibody (66394-1-Ig, Clone: 1F8B9, red).

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  FC experiment of HeLa using 18150-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Podocalyxin antibody (18150-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu rénal humain, cellules HEK-293, cellules HepG2
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu rénal humain,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:500-1:4000
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

18150-1-AP cible Podocalyxin dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Podocalyxin Protéine recombinante Ag12844
• Nom complet: podocalyxin-like
• Masse moléculaire calculée: 526 aa, 55 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC093730
• Symbole du gène: PODXL
• Identification du gène (NCBI): 5420
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.; Authors - Federica Morani
human	IF	Tissue Eng Part A; Genome engineering of human urine-derived stem cells to express lactoferrin and deoxyribonuclease; Authors - Zara Kenigsberg
	WB	Int J Cardiol; Brg1 deficiency in vascular endothelial cells blocks neutrophil recruitment and ameliorates cardiac ischemia-reperfusion injury in mice.; Authors - Xinjian Zhang
human	WB	Sci Rep; Podocyte-specific Crb2 knockout mice develop focal segmental glomerulosclerosis.; Authors - Akiko Tanoue
human	FC	Sci Rep; Urinary extracellular vesicles and micro-RNA as markers of acute kidney injury after cardiac surgery.; Authors - Douglas Miller
human	IF	STAR Protoc; Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells.; Authors - Julie Bejoy

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