Anticorps Polyclonal de lapin anti-POLDIP2

POLDIP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 15080-1-AP

Synonymes

HSPC017, p38, PDIP38, POLD4, Polymerase delta-interacting protein 2

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Galerie de données de validation

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  WB analysis using 15080-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15080-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15080-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15080-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15080-1-AP

  IP result of anti-POLDIP2 (IP:15080-1-AP, 4ug; Detection:15080-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of human stomach cancer using 15080-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15080-1-AP (POLDIP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15080-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15080-1-AP (POLDIP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15080-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLDIP2 antibody (15080-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules HepG2, cellules K-562
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

15080-1-AP cible POLDIP2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLDIP2 Protéine recombinante Ag7154
• Nom complet: polymerase (DNA-directed), delta interacting protein 2
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC000655
• Symbole du gène: POLDIP2
• Identification du gène (NCBI): 26073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POLDIP2(Polymerase delta-interacting protein 2) is also named as PDIP38, POLD4. It is as a unique positive regulator of Nox4 via its association with p22phox and together with Nox4, has profound effects on Rhodependent cytoskeletal remodeling. It is also as a regulator of cell division. It consists of 368 amino acids and has a predicted molecular mass of 42 kDa, with a potential signal peptide cleavage site after the first N-terminal 48 residues, which would result in a protein of 37 kDa(PMID:19574552). Endogenous human and rat POLDIP2 localized to the nucleus, plasma membrane, and cytoplasm(PMID:17623671).

Publications

Species	Application	Title
	WB	EMBO J; Poldip2 promotes mtDNA elimination during Drosophila spermatogenesis to ensure maternal inheritance; Authors - Ziming Wang
mouse	WB	Proc Natl Acad Sci U S A; DNA polymerase δ-interacting protein 2 is a processivity factor for DNA polymerase λ during 8-oxo-7,8-dihydroguanine bypass.; Authors - Maga Giovanni G
human	IF	Cell Cycle; PDIP38 is translocated to the spliceosomes/nuclear speckles in response to UV-induced DNA damage and is required for UV-induced alternative splicing of MDM2.; Authors - Agnes Wong
mouse,human	WB	Cells; Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.; Authors - Jana Key
mouse,rat	WB	Life Sci; Poldip2 Aggravates inflammation in diabetic retinopathy by impairing mitophagy via the AMPK/ULK1/Pink1 pathway; Authors - Siyu Lin

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