Anticorps Polyclonal de lapin anti-POLI

• Phare
• Validé par KD/KO

POLI Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 13635-1-AP

Synonymes

DNA polymerase iota, Eta2, POLI, polymerase (DNA directed) iota, RAD30 homolog B, RAD30B, RAD3OB

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Galerie de données de validation

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  WB analysis using 13635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13635-1-AP (POLI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13635-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13635-1-AP (POLI antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13635-1-AP

  IP Result of anti-POLI (IP:13635-1-AP, 4ug; Detection:13635-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 13635-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13635-1-AP (POLI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 13635-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13635-1-AP (POLI antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu cardiaque humain, tissu testiculaire de rat
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

13635-1-AP cible POLI dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, Moustique, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLI Protéine recombinante Ag4563
• Nom complet: polymerase (DNA directed) iota
• Masse moléculaire calculée: 715 aa, 80 kDa
• Poids moléculaire observé: 87 kDa
• Numéro d’acquisition GenBank: BC032662
• Symbole du gène: POLI
• Identification du gène (NCBI): 11201
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mammalian Pol ι owns an unusual combination of properties: it is stimulated by Mn2+ ions, can bypass some DNA lesions and misincorporates "G" opposite template "T" more frequently than incorporates the correct "A." [PMID:21304950] It also was shown to possess dRP lyase activity. In addition, Pol ι is much more efficient in the presence of Mn2+ in comparison to Mg2+. [PMID:17609217]. It has been proposed that POLI involves in immunoglobulin somatic hypermutation (SHM), bypass of deaminated cytosines, several adducts of the purine bases. DNA strand crosslinks and is involved in DNA repair under oxidative stress[PMID:15199127].

Publications

Species	Application	Title
mouse	WB	Oncogene; The error-prone DNA polymerase ι provides quantitative resistance to lung tumorigenesis and mutagenesis in mice.; Authors - M Iguchi
human	WB	Front Oncol; DNA Polymerase Iota Promotes Esophageal Squamous Cell Carcinoma Proliferation Through Erk-OGT-Induced G6PD Overactivation.; Authors - Zhenzi Su ; KD Validated
human	WB,IHC	Cancer Sci; Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma.; Authors - Chao He
mosquito	IF	Insect Mol Biol; Diverse cellular morphologies during lumen maturation in Anopheles gambiae larval salivary glands.; Authors - M Chiu
human	IHC, IF	Int J Clin Exp Pathol; Elevated DNA polymerase iota (Poli) is involved in the acquisition of aggressive phenotypes of human esophageal squamous cell cancer.; Authors - Haoyao Sun
human	WB	Biotechnol Lett; Transient expression and activity of human DNA polymerase iota in loach embryos.; Authors - Makarova Irina V IV