Anticorps Polyclonal de lapin anti-POLR1A

POLR1A Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 20595-1-AP

Synonymes

A190, POLR1A, RNA polymerase I subunit A1, RPA1, RPA194, RPO1 4

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Galerie de données de validation

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  WB analysis of HeLa using 20595-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20595-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20595-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20595-1-AP

  IP result of anti-POLR1A (IP:20595-1-AP, 4ug; Detection:20595-1-AP 1:300) with HeLa cells lysate 2400ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

20595-1-AP cible POLR1A dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLR1A Protéine recombinante Ag14543
• Nom complet: polymerase (RNA) I polypeptide A, 194kDa
• Masse moléculaire calculée: 1720 aa, 195 kDa
• Poids moléculaire observé: 190-195 kDa
• Numéro d’acquisition GenBank: BC017115
• Symbole du gène: POLR1A
• Identification du gène (NCBI): 25885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POLR1A, DNA-directed RNA polymerase I subunit RPA1, is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. RNA polymerase I (Pol I) located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I ranscription. Together with the second largest subunit, it is the catalytic core component of RNA polymerase I that synthesizes ribosomal RNA precursors.

Publications

Species	Application	Title
		Cell; RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination.; Authors - Sijie Liu
human	WB	J Neurosci; Calmodulin Triggers Activity-Dependent rRNA Biogenesis via Interaction with DDX21; Authors - Jia-Lin Yang
human	IP	Cancer Sci; TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC.; Authors - Chaoshen Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anita (Verified Customer) (12-22-2020)

HeLa cell protein extract (5micrograms)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

POLR1A Antibody Western Blot validation (1:1000 dilution) in HeLa cells (Cat no:20595-1-AP)

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