Anticorps Polyclonal de lapin anti-POLR2C

POLR2C Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 13428-1-AP

Synonymes

hRPB33, hsRPB3, POLR2C, RNA polymerase II subunit 3, RNA polymerase II subunit B3, RPB3, RPB31, RPB33

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Galerie de données de validation

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  WB analysis of mouse kidney using 13428-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13428-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13428-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13428-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13428-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13428-1-AP

  IP result of anti-POLR2C (IP:13428-1-AP, 4ug; Detection:13428-1-AP 1:500) with HeLa cells lysate 2000ug.

• 

  IHC staining of human cervical cancer using 13428-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13428-1-AP (POLR2C antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13428-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13428-1-AP (POLR2C antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

13428-1-AP cible POLR2C dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLR2C Protéine recombinante Ag4228
• Nom complet: polymerase (RNA) II (DNA directed) polypeptide C, 33kDa
• Masse moléculaire calculée: 275 aa, 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC028157
• Symbole du gène: POLR2C
• Identification du gène (NCBI): 5432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
mouse	WB	Mol Cell; Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity; Authors - Yuanjun Li
human	WB	Biomed Microdevices; Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria.; Authors - Hao-Peng Lin
mouse		Genome Biol; Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture; Authors - Yongpeng Jiang

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