Anticorps Polyclonal de lapin anti-POLR2D

POLR2D Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 16093-1-AP

Synonymes

DNA-directed RNA polymerase II subunit D, DNA-directed RNA polymerase II subunit RPB4, HSRBP4, HSRPB4, RNA polymerase II RPB4

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Galerie de données de validation

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  WB analysis using 16093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 16093-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 16093-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16093-1-AP

  IP result of anti-POLR2D (IP:16093-1-AP, 4ug; Detection:16093-1-AP 1:800) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse testis using 16093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16093-1-AP (POLR2D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16093-1-AP (POLR2D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules MCF-7, tissu de muscle squelettique de rat, tissu hépatique de rat
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

16093-1-AP cible POLR2D dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLR2D Protéine recombinante Ag8701
• Nom complet: polymerase (RNA) II (DNA directed) polypeptide D
• Masse moléculaire calculée: 142 aa, 16 kDa
• Poids moléculaire observé: 16-20 kDa
• Numéro d’acquisition GenBank: BC017205
• Symbole du gène: POLR2D
• Identification du gène (NCBI): 5433
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
mouse	WB	Mol Cell; Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity; Authors - Yuanjun Li
human	WB	Elife; A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation.; Authors - Jiale Du

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

chang wei (Verified Customer) (02-18-2020)

It works well for western blot of Hela S3 whole cell lysates at 1:1000 dilution with incubation at 4 degree overnight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa S3

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