Anticorps Polyclonal de lapin anti-POLR2H

POLR2H Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 15086-1-AP

Synonymes

hRPB8, hsRPB8, POLR2H, RPABC3, RPB17, RPB8, RPB8 homolog

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Galerie de données de validation

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  WB analysis of HL-60 using 15086-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15086-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15086-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15086-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15086-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15086-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15086-1-AP

  IP result of anti-POLR2H (IP:15086-1-AP, 4ug; Detection:15086-1-AP 1:500) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human breast cancer using 15086-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15086-1-AP (POLR2H antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 15086-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15086-1-AP (POLR2H antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 15086-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15086-1-AP (POLR2H antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules MCF-7, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

15086-1-AP cible POLR2H dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLR2H Protéine recombinante Ag7166
• Nom complet: polymerase (RNA) II (DNA directed) polypeptide H
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC000739
• Symbole du gène: POLR2H
• Identification du gène (NCBI): 5437
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POLR2H, also known as hRPB8, is a 150 amino acid protein, which localizes in nucleolus and is expressed in 228 organ(s), highest expression level in mucosa of transverse colon. POLR2H. POLR2H is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR2H is a common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively

Publications

Species	Application	Title
mouse	WB	Mol Cell; Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity; Authors - Yuanjun Li
human	IHC	Oncol Lett; Identification of the key genes and pathways in prostate cancer.; Authors - Shutong Fan
mouse		Genome Biol; Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture; Authors - Yongpeng Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yuan (Verified Customer) (12-13-2019)

Worked for human Hela cell. Nice staining at nucleoli area.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Hela cell