Anticorps Polyclonal de lapin anti-POM121

• Phare
• Validé par KD/KO

POM121 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (2)

N° de cat : 15645-1-AP

Synonymes

POM121A, NUP121, Nucleoporin Nup121, Nuclear envelope pore membrane protein POM 121A, Nuclear envelope pore membrane protein POM 121

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Galerie de données de validation

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  WB analysis using 15645-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15645-1-AP (POM121 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 15645-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15645-1-AP (POM121 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 15645-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15645-1-AP (POM121 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 15645-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 15645-1-AP (POM121 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HCT 116 using 15645-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using POM121 antibody (15645-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules HCT 116,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Informations sur le produit

15645-1-AP cible POM121 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POM121 Protéine recombinante Ag8177
• Nom complet: POM121 membrane glycoprotein (rat)
• Masse moléculaire calculée: 984 aa, 99 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC008794
• Symbole du gène: POM121
• Identification du gène (NCBI): 9883
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POM121 is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Catalog#15645-1-AP do well in identifying the nuclear envelope in immunofluorescence experiments.

Publications

Species	Application	Title
human	IF	Nature; Heritable transcriptional defects from aberrations of nuclear architecture; Authors - Stamatis Papathanasiou
human	IF	Nat Neurosci; Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway.; Authors - Yen-Chen Lin
human	IF	Dev Cell; A tubule-sheet continuum model for the mechanism of nuclear envelope assembly; Authors - Gengjing Zhao
human	WB	Elife; Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.; Authors - Melissa Kane
human	IHC,WB	Cancer Biomark; POM121 promotes proliferation and metastasis in non-small-cell lung cancer through TGF-β/SMAD and PI3K/AKT pathways; Authors - Lirong Guan ; KD Validated
human	IF	bioRxiv; Altered nuclear envelope homeostasis is a key pathogenic event in C9ORF72-linked ALS/FTD; Authors - Riccardo Sirtori

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

bing (Verified Customer) (08-06-2019)

Works great in Western Blot, I use it for Hela and Vero cells lysate, go dilution 1:5000 in 5% milk.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Hela cells Vero cells

FH

Yen-Chen (Verified Customer) (12-03-2018)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:400 for IF, 1:2000 for WB
• Cell Tissue Type: human neuron

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