Anticorps Monoclonal anti-POMC

POMC Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

10

CloneNo.

3C12C7

N° de cat : 66358-1-Ig

Synonymes

ACTH, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, CLIP, CLIP Lipotropin beta, Corticotropin lipotropin, Gamma LPH Melanotropin beta, LPH, MSH, POC, POMC, Pro opiomelanocortin, proopiomelanocortin

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Galerie de données de validation

• 

  WB analysis of pig brain using 66358-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 66358-1-Ig

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66358-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 66358-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66358-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 66358-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 66358-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pituitary using 66358-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pituitary using 66358-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pituitary using 66358-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 66358-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse pancreas using 66358-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 66358-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using POMC antibody (66358-1-Ig, Clone: 3C12C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 66358-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human POMC (66358-1-Ig, Clone:3C12C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, cellules A375, cellules HEK-293 transfectées, cellules PC-12, tissu cérébral de rat, tissu placentaire humain
• Résultats positifs en IHC: tissu hypophysaire humain, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Informations sur le produit

66358-1-Ig cible POMC dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: POMC Protéine recombinante Ag17311
• Nom complet: proopiomelanocortin
• Masse moléculaire calculée: 267 aa, 29 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC065832
• Symbole du gène: POMC
• Identification du gène (NCBI): 5443
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.

Publications

Species	Application	Title
human	IHC,IF	Elife; Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma.; Authors - Xuebin Zhang
mouse	IF	Biomed Pharmacother; 919 syrup inhibits ROS-mediated leptin-induced anorexia by activating PPARγ and improves gut flora abnormalities.; Authors - Jing-Wei Xing
rat	WB,IF	Sci Rep; Gold nanoclusters eliminate obesity induced by antipsychotics.; Authors - Meng He
mouse	WB,IF	Biochem Biophys Res Commun; Elongator regulates the melanocortin satiety pathway.; Authors - Joseph Walters
rat	IF	Reprod Sci; FoxO1 Regulates Neuropeptide Y and Pro-opiomelanocortin in the Hypothalamus of Rat Offspring Small for Gestational Age.; Authors - Lifang Zhang
pig	WB,IHC	Food Addit Contam Part A Chem Anal; FumDSB can alleviate the inflammatory response induced by fumonisin B1 in growing pigs.; Authors - Quancheng Liu