Anticorps Polyclonal de lapin anti-POPDC3

• Phare
• Validé par KD/KO

POPDC3 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11800-1-AP

Synonymes

POP3, POPDC3, popeye domain containing 3, Popeye protein 3, RP11 99L11.2

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Galerie de données de validation

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  WB analysis of COLO 320 using 11800-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11800-1-AP (POPDC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11800-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11800-1-AP (POPDC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11800-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11800-1-AP (POPDC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11800-1-AP

  IP Result of anti-POPDC3 (IP:11800-1-AP, 3ug; Detection:11800-1-AP 1:1000) with HeLa cells lysate 220ug.

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  IHC staining of mouse heart using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11800-1-AP (POPDC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11800-1-AP (POPDC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11800-1-AP (POPDC3 Antibody) at dilution of 1:50.

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  IHC staining of human placenta using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11800-1-AP (POPDC3 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HEK-293
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cardiaque de souris, tissu de muscle squelettique humain, tissu placentaire humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Informations sur le produit

11800-1-AP cible POPDC3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POPDC3 Protéine recombinante Ag2375
• Nom complet: popeye domain containing 3
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC022323
• Symbole du gène: POPDC3
• Identification du gène (NCBI): 64208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Popeye domain-containing (POPDC) gene family includes BVES/POPDC1, POPDC2 and POPDC3. POPDC proteins are highly evolutionarily conserved transmembrane proteins expressed in embryonic epithelium, heart and muscle. POPDC3 is a 291-amino acid protein containing three putative transmembrane domains. It has been reported that POPDC3 expression is reduced in gastric cancer, suggesting it might play an important role in the progression and metastases of gastric cancer. (PMID: 10882522; 20627872; 22654436)

Publications

Species	Application	Title
human	WB,IHC	Carcinogenesis; Frequent silencing of popeye domain-containing genes, BVES and POPDC3, is associated with promoter hypermethylation in gastric cancer.; Authors - Kim Mirang M ; KD Validated
human	IHC	Front Oncol; Study on differentially expressed genes between stage M and stage MS neuroblastoma; Authors - Yuying Wu
human	IHC	World J Gastroenterol; Reduced Popdc3 expression correlates with high risk and poor survival in patients with gastric cancer.; Authors - Luo Deng D

Produits associés

BVES Polyclonal antibody

12920-1-AP