Anticorps Polyclonal de lapin anti-POT1

POT1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 10581-1-AP

Synonymes

hPot1, POT1

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Galerie de données de validation

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  WB analysis of HeLa using 10581-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10581-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10581-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10581-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10581-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10581-1-AP (POT1 antibody) at dilution of 1:100 (under 25x lens).

• 

  IHC staining of human cervical cancer using 10581-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10581-1-AP (POT1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10581-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10581-1-AP (POT1 Antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC-3
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

10581-1-AP cible POT1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POT1 Protéine recombinante Ag0875
• Nom complet: POT1 protection of telomeres 1 homolog (S. pombe)
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC002923
• Symbole du gène: POT1
• Identification du gène (NCBI): 25913
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protection of telomeres 1(POT1) is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase. We have discovered that POT1 exists in at least three consistently occurring forms; 90, 70 and 45kDa. The unexpected molecular weights of POT1 seem to be associated with SUMO1 and ubiquitin conjugation; the latter occurring at a double lysine residue at 289-KK-290 (.PMID: 24054699)

Publications

Species	Application	Title
human	IHC	Aging (Albany NY); SIRT6 enhances telomerase activity to protect against DNA damage and senescence in hypertrophic ligamentum flavum cells from lumbar spinal stenosis patients.; Authors - Jianwei Chen
human	IP	Cell Cycle; CGGBP1 phosphorylation constitutes a telomere-protection signal.; Authors - Umashankar Singh
human	WB	Sci Rep; Concomitant underexpression of TGFBR2 and overexpression of hTERT are associated with poor prognosis in cervical cancer.; Authors - Hui Yang
human	WB	Front Cell Dev Biol; Telomere Attrition in Induced Pluripotent Stem Cell-Derived Neurons From ALS/FTD-Related C9ORF72 Repeat Expansion Carriers.; Authors - Hayley Robinson
human	WB	J Cancer; Downregulation of Ubiquitin-conjugating Enzyme UBE2D3 Promotes Telomere Maintenance and Radioresistance of Eca-109 Human Esophageal Carcinoma Cells.; Authors - Hui Yang
mouse	IHC	Exp Gerontol; The altered expression of telomerase components and telomere-linked proteins may associate with ovarian aging in mouse.; Authors - Esra Gozde Kosebent