Anticorps Polyclonal de lapin anti-Liprin Alpha 1

• Phare
• Validé par KD/KO

Liprin Alpha 1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

25

N° de cat : 14175-1-AP

Synonymes

LAR interacting protein 1, LIP 1, LIP.1, LIP1, LIPRIN, Liprin alpha 1, PPFIA1

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Galerie de données de validation

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  WB analysis of HeLa using 14175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14175-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14175-1-AP

  IP Result of anti-Liprin alpha 1 (IP:14175-1-AP, 4ug; Detection:14175-1-AP 1:500) with MCF-7 cells lysate 3200ug.

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  IHC staining of human prostate cancer using 14175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14175-1-AP

  Immunofluorescent analysis of HepG2 cells, using PPFIA1 antibody 14175-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HeLa using 14175-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Liprin alpha 1 antibody (14175-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 17 publications below

Informations sur le produit

14175-1-AP cible Liprin Alpha 1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Liprin Alpha 1 Protéine recombinante Ag5381
• Nom complet: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
• Masse moléculaire calculée: 1202 aa, 136 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: BC034046
• Symbole du gène: PPFIA1
• Identification du gène (NCBI): 8500
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Liprin alpha 1, encoded by the PPFIA1 gene, is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. Liprin alpha 1 may regulate the disassembly of focal adhesions and thus help orchestrate cell-matrix interactions (PMID: 7796809). Alternatively spliced transcript variants encoding distinct isoforms have been described.

Publications

Species	Application	Title
human	IF	Nat Commun; Structural basis of liprin-α-promoted LAR-RPTP clustering for modulation of phosphatase activity.; Authors - Xingqiao Xie
human	WB,IF	Nat Commun; PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.; Authors - Giulia Mana ; KD Validated
mouse	IF	Elife; Local activation of focal adhesion kinase orchestrates the positioning of presynaptic scaffold proteins and Ca2+ signalling to control glucose-dependent insulin secretion.; Authors - Dillon Jevon
human	IF	Stem Cells Transl Med; Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix.; Authors - Reena Singh
human	WB	J Cell Sci; Liprin-α1, ERC1 and LL5 identify a polarized, dynamic compartment implicated in cell migration.; Authors - Veronica Astro ; KD Validated
human	IF	Open Biol; The structural basis of the talin-KANK1 interaction that coordinates the actin and microtubule cytoskeletons at focal adhesions; Authors - Xingchen Li

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