Anticorps Polyclonal de lapin anti-Cyclophilin A

• Phare
• Validé par KD/KO

Cyclophilin A Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 10720-1-AP

Synonymes

Cyclophilin A, Cyclosporin A binding protein, CYPA, CYPH, PPIA, PPIase A, Rotamase A

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Galerie de données de validation

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  WB analysis using 10720-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10720-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 10720-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10720-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10720-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10720-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10720-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 10720-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 10720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 10720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 10720-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 10720-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human tonsillitis using 10720-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human tonsillitis using 10720-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 10720-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cyclophilin A antibody (10720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10720-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Cyclophilin A antibody (10720-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HepG2, cellules K-562, cellules NIH/3T3, cellules Raji
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu d'amygdalite humain, tissu placentaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 18 publications below
• IHC: See 9 publications below
• IF: See 6 publications below

Informations sur le produit

10720-1-AP cible Cyclophilin A dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclophilin A Protéine recombinante Ag1120
• Nom complet: peptidylprolyl isomerase A (cyclophilin A)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC005320
• Symbole du gène: PPIA
• Identification du gène (NCBI): 5478
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.

Publications

Species	Application	Title
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
mouse	WB	Sci Adv; CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.; Authors - Sefan Asamitsu
mouse	WB	Brain; Defective cyclophilin A induces TDP-43 proteinopathy: implications for amyotrophic lateral sclerosis and frontotemporal dementia; Authors - Laura Pasetto ; KO Validated
human	WB	Mol Neurodegener; Decoding distinctive features of plasma extracellular vesicles in amyotrophic lateral sclerosis.; Authors - Laura Pasetto
human,mouse	WB,IHC	Inflamm Regen; Testosterone upregulates glial cell line-derived neurotrophic factor (GDNF) and promotes neuroinflammation to enhance glioma cell survival and proliferation; Authors - Kouminin Kanwore
human	WB,IHC,IF	Virol Sin; Cyclophilin A binds to AKT1 and facilitates the tumorigenicity of Epstein-Barr virus by mediating the activation of AKT/mTOR/NF-κB positive feedback loop; Authors - Shuyu Xin ; KO Validated