Anticorps Polyclonal de lapin anti-Cyclophilin B

• Phare
• Validé par KD/KO

Cyclophilin B Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (2)

N° de cat : 11607-1-AP

Synonymes

Cyclophilin B, CYP S1, CYPB, PPIase B, PPIB, Rotamase B, S cyclophilin, SCYLP

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Galerie de données de validation

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  WB analysis of HeLa using 11607-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11607-1-AP

  WB result of Cyclophilin B antibody (11607-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclophilin B transfected HeLa cells.

• 

  WB analysis of Raji using 11607-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11607-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11607-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11607-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11607-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11607-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11607-1-AP

  Immunofluorescent analysis of HeLa cells using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules NIH/3T3, cellules Raji, cellules RAW 264.7, cellules U-937, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de lymphome humain, tissu de cancer du foie humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

11607-1-AP cible Cyclophilin B dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclophilin B Protéine recombinante Ag2161
• Nom complet: peptidylprolyl isomerase B (cyclophilin B)
• Masse moléculaire calculée: 216 aa, 24 kDa
• Poids moléculaire observé: 19-24 kDa
• Numéro d’acquisition GenBank: BC032138
• Symbole du gène: PPIB
• Identification du gène (NCBI): 5479
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the matrue form (about 19 kDa).

Publications

Species	Application	Title
human	WB	Mol Cell; Metabolic Diversity in Human Non-Small Cell Lung Cancer Cells.; Authors - Pei-Hsuan Chen
human	WB,IHC	Am J Hum Genet; PPIB mutations cause severe osteogenesis imperfecta.; Authors - van Dijk Fleur S FS
mouse	WB	Cell Prolif; OGG1 aggravates renal ischemia-reperfusion injury by repressing PINK1-mediated mitophagy; Authors - Fan Zhao
human	WB,IF	Hum Mol Genet; Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes.; Authors - Pyott Shawna M SM
mouse	WB	Cells; HDAC6 Inhibition Alleviates Ischemia- and Cisplatin-Induced Acute Kidney Injury by Promoting Autophagy; Authors - Lang Shi
human,mouse	WB,IF	J Virol; CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells.; Authors - Watanabe Akira A

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ganesh (Verified Customer) (02-07-2024)

Whole cell extracts of HAP1 (human) cells were resolved on a 4-12% Bis-Tris Gel and proteins were transferred onto a nitrocellulose membrane. Membrane was incubated overnight at 4 deg C with cyclophilin antibody (diluted in 5% Milk in TBS-T). I used this antibody as a loading control in this experiment. The signal was very quick on a film using regular ECL substrate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000 dilution
• Cell Tissue Type: HAP1

Cyclophilin B Antibody Western Blot validation (1:5000 dilution dilution) in HAP1 (Cat no:11607-1-AP)

FH

Parijat (Verified Customer) (08-21-2023)

Works well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000