Anticorps Polyclonal de lapin anti-CYPC

• Phare
• Validé par KD/KO

CYPC Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10287-2-AP

Synonymes

Cyclophilin C, CYPC, PPIase C, PPIC, Rotamase C

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Galerie de données de validation

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  WB analysis using 10287-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10287-2-AP (CYPC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10287-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10287-2-AP (CYPC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 10287-2-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 10287-2-AP (CYPC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10287-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10287-2-AP (CYPC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 10287-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10287-2-AP (CYPC Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10287-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10287-2-AP (CYPC Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 10287-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10287-2-AP (CYPC Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse kidney using 10287-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10287-2-AP (CYPC Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 10287-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYPC antibody (10287-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HEK-293 using 10287-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10287-2-AP (CYPC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HuH-7, tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below

Informations sur le produit

10287-2-AP cible CYPC dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYPC Protéine recombinante Ag0209
• Nom complet: peptidylprolyl isomerase C (cyclophilin C)
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC002678
• Symbole du gène: PPIC
• Identification du gène (NCBI): 5480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclophilin C also named as peptidyl-prolyl cis-trans isomerase C(PPIC), is expressed in several bone marrow (BM) hematopoietic progenitors and in T‐cell precursors(PMID: 33864384). CYPC/PPIC catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Similar to other PPIases, this protein can bind immunosuppressant cyclosporin A. In addition, CYPC/PPIC is involved in many biological processes, including mitochondrial metabolism, REDOX and inflammation, as well as related diseases and conditions, such as ischemia-reperfusion injury, AIDS and cancer.

Publications

Species	Application	Title
human	WB	J Biol Chem; The isomerase active site of cyclophilin A is critical for hepatitis C virus replication.; Authors - Udayan Chatterji ; KD Validated
human	WB	J Biol Chem; Depletion of Cyclophilins B and C Leads to Dysregulation of Endoplasmic Reticulum Redox Homeostasis.; Authors - Pawel Stocki
human	WB	PLoS One; Cyclophilin C Participates in the US2-Mediated Degradation of Major Histocompatibility Complex Class I Molecules.; Authors - Daniel C Chapman
human	WB	Int J Mol Sci; Pharmacological Targeting of the ER-Resident Chaperones GRP94 or Cyclophilin B Induces Secretion of IL-22 Binding Protein Isoform-1 (IL-22BPi1).; Authors - Paloma Gómez-Fernández
human	WB	ACS Chem Neurosci; Furanoditerpenes from Spongia (Spongia) tubulifera Display Mitochondrial-Mediated Neuroprotective Effects by Targeting Cyclophilin D.; Authors - Rebeca Alvariño
mouse	WB	Front Physiol; Extracellular cyclophilins A and C induce dysfunction of pancreatic microendothelial cells; Authors - Rebeca Alvariño