Anticorps Polyclonal de lapin anti-PPID

PPID Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 12716-1-AP

Synonymes

Cyclophilin 40, Cyclophilin D, Cyclophilin related protein, CYP 40, CYP40, CYPD, peptidylprolyl isomerase D, PPIase D, PPID, Rotamase D

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Galerie de données de validation

• 

  WB analysis of mouse brain using 12716-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12716-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12716-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12716-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12716-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12716-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12716-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12716-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12716-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12716-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12716-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12716-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12716-1-AP

  IP Result of anti-PPID (IP:12716-1-AP, 4ug; Detection:12716-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human cervical cancer using 12716-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12716-1-AP (PPID antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse testis using 12716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12716-1-AP (PPID antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12716-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12716-1-AP (PPID antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 12716-1-AP

  1X10^6 HeLa cells were stained with 0.2ug PPID antibody (12716-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules Raji, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

12716-1-AP cible PPID dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPID Protéine recombinante Ag3409
• Nom complet: peptidylprolyl isomerase D
• Masse moléculaire calculée: 370 aa, 41 kDa
• Poids moléculaire observé: 38-41 kDa
• Numéro d’acquisition GenBank: BC030707
• Symbole du gène: PPID
• Identification du gène (NCBI): 5481
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).

Publications

Species	Application	Title
rat	WB,CoIP	Cardiovasc Toxicol; PM2.5-Induced Programmed Myocardial Cell Death via mPTP Opening Results in Deteriorated Cardiac Function in HFpEF Mice.; Authors - Tingting Wu
human	WB	Mol Med Rep; Downregulation of microRNA‑199a‑5p attenuates hypoxia/reoxygenation‑induced cytotoxicity in cardiomyocytes by targeting the HIF‑1α‑GSK3β‑mPTP axis.; Authors - Da-Wei Liu
human	WB	Eur J Pharmacol; A novel ent-kaurane diterpenoid analog, DN3, selectively kills human gastric cancer cells via acting directly on mitochondria.; Authors - Yong-Cheng Ma
human	WB	Dev Cell; Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome; Authors - Ji Geng
rat	WB,IF	Bone Res; Sensory nerves directly promote osteoclastogenesis by secreting peptidyl-prolyl cis-trans isomerase D (Cyp40); Authors - Junqin Li
mouse,rat	WB,CoIP	J Hazard Mater; T-2 toxin induces mitochondrial dysfunction in chondrocytes via the p53-cyclophilin D pathway; Authors - Fang-Fang Yu