Anticorps Recombinant de lapin anti-PPID

PPID Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241361B2

N° de cat : 84115-1-RR

Synonymes

Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase

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Galerie de données de validation

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  WB analysis using 84115-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84115-1-RR (PPID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using 84115-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug PPID Recombinant Antibody (84115-1-RR, Clone:241361B2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84115-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84115-1-RR against Human PPID were performed. The affinity constant is 0.313 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Jurkat, cellules MCF7
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84115-1-RR cible PPID dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PPID Protéine recombinante Ag3409
• Nom complet: peptidylprolyl isomerase D
• Masse moléculaire calculée: 370 aa, 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC030707
• Symbole du gène: PPID
• Identification du gène (NCBI): 5481
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).

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