Anticorps Polyclonal de lapin anti-PPIF

PPIF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 18466-1-AP

Synonymes

PPIF-Specific, Cyclophilin D, Cyclophilin F, Cyp D, CyP-D

---

Galerie de données de validation

• 

  WB analysis using 18466-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18466-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 18466-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 18466-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 18466-1-AP

  IP result of anti-PPIF (IP:18466-1-AP, 3ug; Detection:18466-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human heart using 18466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (PPIF Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 18466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (CYPA Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 18466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18466-1-AP (PPIF-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18466-1-AP (PPIF-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 18466-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PPIF antibody 18466-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

18466-1-AP cible PPIF dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPIF Protéine recombinante Ag13151
• Nom complet: peptidylprolyl isomerase F
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC005020
• Symbole du gène: PPIF
• Identification du gène (NCBI): 10105
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPIF, also named as Cyclophilin D (CypD), CYP3, PPIase F and Rotamase F, belongs to the cyclophilin-type PPIase family. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is a mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore. This antibody is immunized with full length gene PPIF and absorbed by PPIA. So this antibody is specific to PPIF.

Publications

Species	Application	Title
mouse	WB	Nat Commun; The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury; Authors - Hakjoo Lee
human	IHC	Aging (Albany NY); Overexpression of TICRR and PPIF confer poor prognosis in endometrial cancer identified by gene co-expression network analysis.; Authors - Linlin Yang
mouse	WB	J Biol Chem; The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.; Authors - Hakjoo Lee
human	IF	PLoS One; Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM.; Authors - Wang Yun E YE
mouse	WB	EMBO J; Prohibitin 1 regulates mtDNA release and downstream inflammatory responses; Authors - Hao Liu
human	WB	Biol Pharm Bull; Cinnamtannin B-1 inhibits the progression of osteosarcoma by regulating the miR-1281/PPIF axis; Authors - Jun Jia

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Veronica (Verified Customer) (01-30-2024)

Really satisfied with the result in WB. No aspecific signal, clear band. Reccomended! Conditions: 12% acrylammide gel Ab’: 1:2000 in 5% milk O/N

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa

PPIF Antibody Western Blot validation (1:2000 dilution) in HeLa (Cat no:18466-1-AP)

{{ptg:RelatedPrimaryAntibodies}}