Anticorps Polyclonal de lapin anti-PPM1B

PPM1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA, Cell treatment

Conjugaison

Non conjugué

Publications(6)

N° de cat : 13193-1-AP

Synonymes

Protein phosphatase 1B, PP2C-beta, PP2CB, PP2C beta, EC:3.1.3.16

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Galerie de données de validation

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  WB analysis using 13193-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13193-1-AP (PPM1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13193-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13193-1-AP (PPM1B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 13193-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13193-1-AP (PPM1B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skeletal muscle using 13193-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13193-1-AP (PPM1B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 13193-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13193-1-AP (PPM1B Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 13193-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 13193-1-AP (PPM1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below
• IP: See 2 publications below

Informations sur le produit

13193-1-AP cible PPM1B dans les applications de WB, IHC, IF/ICC, IP, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPM1B Protéine recombinante Ag3871
• Nom complet: protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
• Masse moléculaire calculée: 479 aa, 53 kDa
• Poids moléculaire observé: 36, 53 kDa
• Numéro d’acquisition GenBank: BC012002
• Symbole du gène: PPM1B
• Identification du gène (NCBI): 5495
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPM1B is a member of the metal-dependent serine/threonine protein phosphatase (PPM) family with important regulatory functions in cellular signalling pathways(PMID: 18066953). PPM1B is also an important negative regulator of several stress signalling pathways and has been shown to negatively regulate the stress-activated p38 and JNK pathways by directly dephosphorylating upstream signalling proteins(PMID: 9824284). Moreover, PPM1B has also been shown to prevent cell death by negatively regulating necrotic cell death in a RIP3 dephosphorylation-dependent manner.(PMID: 25751141) PPM1B can be detected as the WM of 36-55 kDa.

Publications

Species	Application	Title
human	IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li
mouse	IP,IF,Cell treatment	Cell Rep; Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis; Authors - Katyayanee Neopane
human		Biochim Biophys Acta Mol Cell Res; TXLNA enhances TBK1 phosphorylation by suppressing PPM1B recruitment; Authors - Xiao Tian
mouse		Front Pharmacol; Initial Characterization of Stressed Transgenic Mice With Cardiomyocyte-Specific Overexpression of Protein Phosphatase 2C.; Authors - Paula Bollmann
human	IF	Exp Cell Res; RBM10 regulates the tumorigenic potential of human cancer cells by modulating PPM1B and YBX1 activities; Authors - Yueyang Li
human		Front Pharmacol; Icariin promotes functional recovery in rats after spinal cord injury by inhibiting YAP and regulating PPM1B ubiquitination to inhibiting the activation of reactive astrocytes; Authors - Sa Feng

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