Anticorps Polyclonal de lapin anti-PPM1K

• Phare
• Validé par KD/KO

PPM1K Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (2)

N° de cat : 14573-1-AP

Synonymes

PP2C kappa, PP2Cm, PPM1K, PTMP, UG0882E07

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Galerie de données de validation

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  WB analysis of human brain using 14573-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14573-1-AP (PPM1K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14573-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14573-1-AP (PPM1K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 14573-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14573-1-AP (PPM1K antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 14573-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14573-1-AP (PPM1K antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cardiaque humain
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

14573-1-AP cible PPM1K dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPM1K Protéine recombinante Ag5660
• Nom complet: protein phosphatase 1K (PP2C domain containing)
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 30-40 kDa
• Numéro d’acquisition GenBank: BC037552
• Symbole du gène: PPM1K
• Identification du gène (NCBI): 152926
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein phosphatase, Mg2+/Mn2+ dependent 1K (PPM1K), is a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. PPMiK, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore.PPM1K is essential for the regulation and nutrient-induced activation of the branched-chain alpha-keto acid dehydrogenase complex (BCKD or BCKDC), which catalyzes the breakdown of branched-chain amino acids (BCAA).

Publications

Species	Application	Title
human,mouse	WB,IF	Nat Commun; Glucose promotes cell growth by suppressing branched-chain amino acid degradation.; Authors - Dan Shao ; KO Validated
mouse	WB	Free Radic Biol Med; Elevated branched-chain α-keto acids exacerbate macrophage oxidative stress and chronic inflammatory damage in type 2 diabetes mellitus.; Authors - Shuyun Liu
mouse	WB	Front Pharmacol; Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice.; Authors - Yang Yang
rat	WB	Physiol Rep; Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts; Authors - Toshifumi Ogawa

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

CHAO (Verified Customer) (01-30-2020)

Clear, sharp band as predicted size 40 kDa.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

FH

Lorenza (Verified Customer) (11-08-2019)

I have tried four different antibodies from different companies and this is the only one that works in total lysate (and not only in isolated mitochondria). All the others give an unspecific band at the same molecular weight. Works perfectly. There are some unspecific bands at higher molecular weights but the right bands is easily detectable.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: total heart lysate and total liver lysate

PPM1K Antibody Western Blot, validation (1:1000 dilution) in total heart lysate and total liver lysate (Cat no:14573-1-AP)