Anticorps Monoclonal anti-PPP1CA

• Phare
• Validé par KD/KO

PPP1CA Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, poisson-zèbre, porc, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

CloneNo.

1C10A2

N° de cat : 67070-1-Ig

Synonymes

EC:3.1.3.16, PP 1A, PP-1A, PPP1A, Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

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Galerie de données de validation

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  WB analysis using 67070-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67070-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of zebrafish using 67070-1-Ig

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67070-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 67070-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules T-47D, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal, tissu de poisson-zèbre

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IP: See 1 publications below

Informations sur le produit

67070-1-Ig cible PPP1CA dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, poisson-zèbre, porc, rat, souris

• Réactivité: Humain, Lapin, poisson-zèbre, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPP1CA Protéine recombinante Ag28451
• Nom complet: protein phosphatase 1, catalytic subunit, alpha isoform
• Masse moléculaire calculée: 330 aa, 38 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC001888
• Symbole du gène: PPP1CA
• Identification du gène (NCBI): 5499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166).

Publications

Species	Application	Title
mouse	WB	Nat Commun; Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice; Authors - Wen Yin
human	WB,IP	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang ; KD Validated
mouse	WB	J Neurosci; CREB inactivation by HDAC1/PP1γ contributes to dopaminergic neurodegeneration in Parkinson's disease.; Authors - Xiaoyi Xu 许潇依
rat	WB	Neurotoxicology; Nuclear accumulation of histone deacetylase 4 (HDAC4) by PP1-mediated dephosphorylation exerts neurotoxicity in Pb-exposed neural cells.; Authors - Xiaozhen Gu
human	WB	Exp Ther Med; Small interfering RNA-mediated knockdown of KRT80 suppresses colorectal cancer proliferation.; Authors - Jiatian Lin
mouse,human	WB	Zool Res; EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50; Authors - Kai-Qin Li ; KD Validated

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