Anticorps Polyclonal de lapin anti-PPP1CB-Specific

PPP1CB-Specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF,ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10140-2-AP

Synonymes

PP1 Beta, PP 1B, PPP1CB, PPP1CD

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Galerie de données de validation

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  WB analysis of human brain using 10140-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10140-2-AP (PPP1CB-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10140-2-AP

  NIH3T3 cells were subjected to SDS PAGE followed by western blot with 10140-2-AP (PPP1CB-Specific antibody) at dilution of 1:1500.

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  IP experiment of mouse brain using 10140-2-AP

  IP Result of anti-PPP1CB-Specific (IP:10140-2-AP, 4ug; Detection:10140-2-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IP experiment of mouse brain using 10140-2-AP

  IP Result of anti-PPP1CB-Specific (IP:10140-2-AP, 4ug; Detection:10140-2-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human prostate cancer using 10140-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10140-2-AP (PPP1CB-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of NIH/3T3 using 10140-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 10140-2-AP (PPP1CB-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, NIH/3T3
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

10140-2-AP cible PPP1CB-Specific dans les applications de WB, IP, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP1CB-Specific Protéine recombinante Ag0183
• Nom complet: protein phosphatase 1, catalytic subunit, beta isoform
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC002697
• Symbole du gène: PPP1CB
• Identification du gène (NCBI): 5500
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein phosphatase 1 (PP1) is the main eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular processes including protein-protein interactions, gene transcription, cell-cycle progression and apoptosis. It is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. The catalytic subunit of PP1 (PP1c) can interact with amount of regulatory subunits in vivo that target it to particular subcellular locations and regulate its activity towards specific substrates. PPP1CB migrated as a 37kd protein on SDS page. This antibody is specific to PPP1CB.

Publications

Species	Application	Title
human	WB	Am J Physiol Cell Physiol; PP1γ functionally augments the alternative splicing of CaMKIIδ through interacting with ASF.; Authors - Chao Huang
human	WB,IHC	Melanoma Res; Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma.; Authors - Sun Daxin D
rat	WB, IP	Clin Exp Pharmacol Physiol; Rescue of cardiac failing and remodeling by inhibition of PP1γ is associated with suppression of the ASF-mediated splicing of CaMKIIδ; Authors - Ru-Jia Liao