Anticorps Polyclonal de lapin anti-PPP1CB-Specific

• Phare
• Validé par KD/KO

PPP1CB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 55136-1-AP

Synonymes

PPP1CB, PPP1CD, PP-1B, PP1 Beta, PP 1B

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Galerie de données de validation

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  WB analysis using 55136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 55136-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 55136-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules BxPC-3, cellules NIH/3T3, cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de la peau humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

55136-1-AP cible PPP1CB-Specific dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein phosphatase 1, catalytic subunit, beta isoform
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 33-40 kDa
• Numéro d’acquisition GenBank: NM_002709
• Symbole du gène: PPP1CB
• Identification du gène (NCBI): 5500
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP1CB, also named as PP-1B, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CB is involved in regulation of ionic conductances and long-term synaptic plasticity. This antibody is specific to PPP1CB.

Publications

Species	Application	Title
mouse	WB	J Adv Res; Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DDX5-ATF1-PPP2R5A signaling; Authors - Di Fan
human	WB	PLoS Pathog; Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids.; Authors - Zhanying Hu ; KD Validated
human	WB	Exp Eye Res; PPP1CA/YAP/GS/Gln/mTORC1 pathway activates retinal Müller cells during diabetic retinopathy.; Authors - Yang Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Balawant (Verified Customer) (08-23-2022)

I have used this antibody for western blot in mice tissue sample (colon tissue). it is working great.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Colon tissue sample and caco2 cells

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