Anticorps Polyclonal de lapin anti-PPP1CC

PPP1CC Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 11082-1-AP

Synonymes

PP1 Gamma, PP 1G, PPP1CC, PPP1G

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Galerie de données de validation

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  WB analysis using 11082-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11082-1-AP (PPP1CC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11082-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11082-1-AP (PPP1CC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 11082-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11082-1-AP (PPP1CC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 11082-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 11082-1-AP (PPP1CC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11082-1-AP

  IP Result of anti-PPP1CC (IP:11082-1-AP, 4ug; Detection:11082-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human pancreas cancer using 11082-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11082-1-AP (PPP1CC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11082-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11082-1-AP (PPP1CC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 11082-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 11082-1-AP (PPP1CC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HEK-293T, cellules L02, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IP: See 2 publications below

Informations sur le produit

11082-1-AP cible PPP1CC dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP1CC Protéine recombinante Ag1550
• Nom complet: protein phosphatase 1, catalytic subunit, gamma isoform
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC014073
• Symbole du gène: PPP1CC
• Identification du gène (NCBI): 5501
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.

Publications

Species	Application	Title
human	WB	J Biol Chem; Dephosphorylation activates the interferon-stimulated schlafen family member 11 in the DNA Damage Response.; Authors - Dane Malone
human	WB	Am J Physiol Cell Physiol; PP1γ functionally augments the alternative splicing of CaMKIIδ through interacting with ASF.; Authors - Chao Huang
rat	WB, IP	Clin Exp Pharmacol Physiol; Rescue of cardiac failing and remodeling by inhibition of PP1γ is associated with suppression of the ASF-mediated splicing of CaMKIIδ; Authors - Ru-Jia Liao
human	WB	Cell Death Dis; Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration; Authors - Xiaoman Zhu
human,mouse	WB,IP	Dev Cell; Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma; Authors - Yan Gong ; KD Validated
human,mouse	WB	Zool Res; EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50; Authors - Kai-Qin Li ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jua (Verified Customer) (08-01-2018)

• Applications: Flow Cytometry,
• Primary Antibody Dilution: Souris têtard isralien