Anticorps Polyclonal de lapin anti-PPP1CC

PPP1CC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 55150-1-AP

Synonymes

Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP-1G, EC:3.1.3.16

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Galerie de données de validation

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  WB analysis using 55150-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55150-1-AP (PPP1CC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 55150-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55150-1-AP (PPP1CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55150-1-AP

  IP result of anti-PPP1CC (IP:55150-1-AP, 4ug; Detection:55150-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IHC staining of human heart using 55150-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55150-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 55150-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 55150-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 55150-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PPP1CC antibody (55150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules L02, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

55150-1-AP cible PPP1CC dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein phosphatase 1, catalytic subunit, gamma isoform
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: NM_002710
• Symbole du gène: PPP1CC
• Identification du gène (NCBI): 5501
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. The antibody is specific to PPP1CC.

Publications

Species	Application	Title
mouse	WB	Front Cell Dev Biol; PERK Signaling Controls Myoblast Differentiation by Regulating MicroRNA Networks.; Authors - Ye-Ya Tan
mouse	WB	J Biol Chem; Hydrogen sulfide modulates eukaryotic translation initiation factor 2α (eIF2α) phosphorylation status in the integrated stress response pathway.; Authors - Vinita Yadav
human	WB	Cell Death Dis; Genome-wide identification of microRNA targets reveals positive regulation of the Hippo pathway by miR-122 during liver development.; Authors - Yin Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vinita (Verified Customer) (12-04-2018)

• Applications: Western Blot,
• Cell Tissue Type: Hek293

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