Anticorps Monoclonal anti-PPP1CC

PPP1CC Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2G11C3

N° de cat : 68122-1-Ig

Synonymes

2G11C3, EC:3.1.3.16, PP-1G, Serine/threonine-protein phosphatase PP1-gamma catalytic subunit

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Galerie de données de validation

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  WB analysis using 68122-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68122-1-Ig (PPP1CC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IF Staining of C2C12 using 68122-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using PPP1CC antibody (68122-1-Ig, Clone: 2G11C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 68122-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PPP1CC antibody (68122-1-Ig, Clone: 2G11C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules HeLa, cellules C2C12

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68122-1-Ig cible PPP1CC dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPP1CC Protéine recombinante Ag32348
• Nom complet: protein phosphatase 1, catalytic subunit, gamma isoform
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC014073
• Symbole du gène: PPP1CC
• Identification du gène (NCBI): 5501
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.

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