Anticorps Polyclonal de lapin anti-CPI17

CPI17 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 11658-1-AP

Synonymes

CPI 17, CPI17, PPP1INL, PPP1R14A

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Galerie de données de validation

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  WB analysis of MCF-7 using 11658-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11658-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11658-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11658-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11658-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 11658-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11658-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 11658-1-AP

  Immunofluorescent analysis of PC-3 cells using 11658-1-AP (CPI17 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, cellules HepG2, cellules Jurkat, cellules PC-3, tissu de côlon humain
• Résultats positifs en IF: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

11658-1-AP cible CPI17 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPI17 Protéine recombinante Ag2237
• Nom complet: protein phosphatase 1, regulatory (inhibitor) subunit 14A
• Masse moléculaire calculée: 147 aa, 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC021089
• Symbole du gène: PPP1R14A
• Identification du gène (NCBI): 94274
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP1R14A, also named as CPI17, belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. CPI17 has two isoforms:CPI-17alpha (17 kDa) and CPI-17beta (13 kDa).