Anticorps Polyclonal de lapin anti-PPP1R15B

• Phare
• Validé par KD/KO

PPP1R15B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (2)

N° de cat : 14634-1-AP

Synonymes

CREP, PPP1R15B

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Galerie de données de validation

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  WB analysis using 14634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 14634-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14634-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14634-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14634-1-AP

  IP Result of anti-PPP1R15B;CReP (IP:14634-1-AP, 3ug; Detection:14634-1-AP 1:1000) with MCF-7 cells lysate 2500ug.

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  IHC staining of mouse brain using 14634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14634-1-AP (PPP1R15B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 14634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14634-1-AP (PPP1R15B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14634-1-AP

  Immunofluorescent analysis of HepG2 cells using 14634-1-AP (PPP1R15B antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG .

• 

  IF Staining of SH-SY5Y using 14634-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP1R15B antibody (14634-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules HEK-293, cellules MCF-7, cellules MDA-MB-231, cellules SGC-7901, cellules SKOV-3
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 19 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14634-1-AP cible PPP1R15B dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP1R15B Protéine recombinante Ag6213
• Nom complet: protein phosphatase 1, regulatory (inhibitor) subunit 15B
• Masse moléculaire calculée: 79 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC065280
• Symbole du gène: PPP1R15B
• Identification du gène (NCBI): 84919
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).

Publications

Species	Application	Title
human	WB	Cell; Target-Based Discovery of an Inhibitor of the Regulatory Phosphatase PPP1R15B.; Authors - Agnieszka Krzyzosiak
mouse	WB	Neuron; Excitatory neuron-specific suppression of the integrated stress response contributes to autism-related phenotypes in fragile X syndrome; Authors - Mehdi Hooshmandi
mouse	WB	Hepatology; PMID: 29698569; Authors - Xu Xu ; KO Validated
human	WB,IF	Sci Adv; CReP mediates selective translation initiation at the endoplasmic reticulum.; Authors - Jonathan P Kastan
mouse	WB	Nat Commun; Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.; Authors - Hongtan Wu
mouse	WB	Proc Natl Acad Sci U S A; An inhibitor of HIV-1 protease modulates constitutive eIF2α dephosphorylation to trigger a specific integrated stress response.; Authors - Aude De Gassart

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

Detects a major band ~100 kDa and shows upregulation in stressed cells (MG132 treatment). WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~55kDa. Also was tested in IHC in mouse CNS tissue and in ICC in human cells; the signal appears specific in both cases (not shown).

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, SH-SY5Y cells; mouse, brain

PPP1R15B Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in Human, SH-SY5Y cells; mouse, brain (Cat no:14634-1-AP)

FH

Will (Verified Customer) (10-24-2019)

good antibody, recommended by a colleague, would order again

• Applications: Western Blot,
• Cell Tissue Type: hepatocytes

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