Anticorps Polyclonal de lapin anti-PPP2CA

• Phare
• Validé par KD/KO

PPP2CA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 13482-1-AP

Synonymes

PP2A alpha, PP2Ac, PP2CA, PPP2CA, Replication protein C, RP C

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Galerie de données de validation

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  WB analysis using 13482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13482-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13482-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13482-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 13482-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 13482-1-AP

  IP Result of anti-PPP2CA (IP:13482-1-AP, 4ug; Detection:13482-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human liver using 13482-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 13482-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 13482-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP2CA antibody (13482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of MCF-7 using 13482-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PPP2CA (13482-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules Jurkat, cellules MCF-7, tissu hépatique humain
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 27 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

13482-1-AP cible PPP2CA dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP2CA Protéine recombinante Ag4294
• Nom complet: protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
• Masse moléculaire calculée: 309 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC031696
• Symbole du gène: PPP2CA
• Identification du gène (NCBI): 5515
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

Publications

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
human	WB,IP	Cell Res; Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.; Authors - Xiang Xu ; KD Validated
human	WB	Acta Pharm Sin B; WSB1 regulates c-Myc expression through β-catenin signaling and forms a feedforward circuit.; Authors - Xiaomeng Gao
mouse	WB	Cardiovasc Res; Role of type 2A phosphatase regulatory subunit B56α in regulating cardiac responses to β-adrenergic stimulation in vivo.; Authors - Sarah-Lena Puhl
mouse	WB	J Pineal Res; Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload; Authors - Xinghua Qin
human	IP	Redox Biol; Sustained IKKβ phosphorylation and NF-κB activation by superoxide-induced peroxynitrite-mediated nitrotyrosine modification of B56γ3 and PP2A inactivation.; Authors - Yi Hui Yee