Anticorps Recombinant de lapin anti-PPP2CA

PPP2CA Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241112F3

N° de cat : 84155-6-RR

Synonymes

241112F3, EC:3.1.3.16, PP2A alpha, PP2A-alpha, PP2Ac

---

Galerie de données de validation

• 

  IF Staining of MCF-7 using 84155-6-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP2CA antibody (84155-6-RR, Clone: 241112F3 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HeLa using 84155-6-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PPP2CA Recombinant antibody (84155-6-RR, Clone:241112F3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 84155-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84155-6-RR against Human PPP2CA were performed. The affinity constant is 24.4 nM.

---

Applications testées

• Résultats positifs en IF/ICC: cellules MCF-7,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84155-6-RR cible PPP2CA dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PPP2CA Protéine recombinante Ag4294
• Nom complet: protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
• Masse moléculaire calculée: 309 aa, 36 kDa
• Numéro d’acquisition GenBank: BC031696
• Symbole du gène: PPP2CA
• Identification du gène (NCBI): 5515
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

{{ptg:RelatedPrimaryAntibodies}}