Anticorps Polyclonal de lapin anti-PPP2R1A

• Phare
• Validé par KD/KO

PPP2R1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (2)

N° de cat : 15882-1-AP

Synonymes

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, PR65A, PP2A subunit A isoform R1-alpha, PP2A subunit A isoform PR65-alpha, Medium tumor antigen-associated 61 kDa protein

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Galerie de données de validation

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  WB analysis using 15882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15882-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 15882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 15882-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15882-1-AP

  IP result of anti-PPP2R1A (IP:15882-1-AP, 4ug; Detection:15882-1-AP 1:300) with HepG2 cells lysate 3200ug.

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  IHC staining of human kidney using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse kidney using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15882-1-AP (PPP2R1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15882-1-AP (PPP2R1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 15882-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PPP2R1A antibody (15882-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HEK-293 using 15882-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PPP2R1A antibody (15882-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules L02, cellules SMMC-7721, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu rénal de souris, tissu de tumeur ovarienne humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

15882-1-AP cible PPP2R1A dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP2R1A Protéine recombinante Ag8661
• Nom complet: protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform
• Masse moléculaire calculée: 589 aa, 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC001537
• Symbole du gène: PPP2R1A
• Identification du gène (NCBI): 5518
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP2R1A belongs to the phosphatase 2A regulatory subunit A family. PPP2R1A encodes the α-isoform of the scaffolding subunit of the serine/threonine protein phosphatase 2A (PP2A) holoenzyme. This tumour suppressor complex is involved in growth and survival pathways and PPP2R1A mutations in endometrial high-grade serous carcinomas are a frequent and potentially targetable feature of this disease(PMID: 21381030). The PPP2R1A gene has 86% identity with the PPP2R1B gene(PMID:7681822).

Publications

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
human	WB	Mol Cell; SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.; Authors - Shibin Hu
human	WB	Front Cell Dev Biol; BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.; Authors - Baoyu Chen
human	WB	Cancer Cell Int; PPP2R1A silencing suppresses LUAD progression by sensitizing cells to nelfinavir-induced apoptosis and pyroptosis; Authors - Yating Liu ; KD Validated
human	WB,IHC	Cell Rep; IMPDH2 dephosphorylation under FGFR signaling promotes S-phase progression and tumor growth; Authors - Bei Zhou
		J Virol; Classical swine fever virus NS5A protein activates autophagy via the PP2A-DAPK3-Beclin 1 axis; Authors - Jinfu Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wissem (Verified Customer) (07-30-2024)

antibody works too detect PPP2R1A in HEK293 and U2OS cell extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

FH

Zee (Verified Customer) (01-28-2020)

It worked very well for western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

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