Anticorps Polyclonal de lapin anti-PPP2R1A

PPP2R1A Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 15882-1-AP

Synonymes

PPP2R1A, PR65A

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Galerie de données de validation

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  WB analysis of mouse kidney using 15882-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 15882-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 15882-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15882-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15882-1-AP

  IP Result of anti-PPP2R1A (IP:15882-1-AP, 4ug; Detection:15882-1-AP 1:300) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human kidney using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de rat, cellules A431, cellules HepG2, cellules L02, cellules SMMC-7721, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu rénal humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• CoIP: See 2 publications below

Informations sur le produit

15882-1-AP cible PPP2R1A dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP2R1A Protéine recombinante Ag8661
• Nom complet: protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform
• Masse moléculaire calculée: 589 aa, 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC001537
• Symbole du gène: PPP2R1A
• Identification du gène (NCBI): 5518
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP2R1A belongs to the phosphatase 2A regulatory subunit A family. PPP2R1A encodes the α-isoform of the scaffolding subunit of the serine/threonine protein phosphatase 2A (PP2A) holoenzyme. This tumour suppressor complex is involved in growth and survival pathways and PPP2R1A mutations in endometrial high-grade serous carcinomas are a frequent and potentially targetable feature of this disease(PMID: 21381030). The PPP2R1A gene has 86% identity with the PPP2R1B gene(PMID:7681822).

Publications

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
human	WB	Mol Cell; SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.; Authors - Shibin Hu
human	WB	Front Cell Dev Biol; BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.; Authors - Baoyu Chen
human,pig	WB,CoIP	Microbiol Spectr; TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells; Authors - Changsheng Jiang ; KD Validated
human	CoIP	Dev Cell; Fam72a functions as a cell-cycle-controlled gene during proliferation and antagonizes apoptosis through reprogramming PP2A substrates; Authors - Yuan Fu
human,mouse	WB	Cell Death Discov; Venlafaxine antagonizes the noradrenaline-promoted colon cancer progression by inhibiting the norepinephrine transporter; Authors - Huahua Zhang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zee (Verified Customer) (01-28-2020)

It worked very well for western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue