Anticorps Polyclonal de lapin anti-PPP2R2B/A/C/D

PPP2R2B/A/C/D Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 13123-1-AP

Synonymes

B55 BETA, B55β, PP2A B55BETA, PP2A PR55B, PP2A subunit B isoform beta, PP2A subunit B isoform R2 beta, PP2AB BETA, PP2APR55 BETA, PPP2R2B, PPP2R2B/A/C/D, PR2AB BETA, PR2AB55 BETA, PR2APR55 BETA, PR52B, PR55 BETA, SCA12

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Galerie de données de validation

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  WB analysis using 13123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 13123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13123-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13123-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 13123-1-AP

  IP Result of anti-PPP2R2B/A/C/D (IP:13123-1-AP, 4ug; Detection:13123-1-AP 1:500) with SH-SY5Y cells lysate 1200ug.

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  IHC staining of mouse brain using 13123-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 13123-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP2R2B/A/C/D antibody (13123-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal
• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13123-1-AP cible PPP2R2B/A/C/D dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP2R2B/A/C/D Protéine recombinante Ag3860
• Nom complet: protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
• Masse moléculaire calculée: 443 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC031790
• Symbole du gène: PPP2R2B
• Identification du gène (NCBI): 5521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. This antibody has cross-reactivity to PPP2R2A, PPP2R2C and PPP2R2D.

Publications

Species	Application	Title
human	WB,CoIP	Mol Ther Nucleic Acids; miR21 modulates the Hippo signaling pathway via interference with PP2A Bβ to inhibit trophoblast invasion and cause preeclampsia; Authors - Mingyu Hu
human	WB,IHC,IF	Cancer Cell Int; PPP2R2B downregulation is associated with immune evasion and predicts poor clinical outcomes in triple-negative breast cancer.; Authors - Zheng Li